Mycobacteria Flashcards
Mycpbacterium cell wall composition
N-glycocylmuramic acid
Droplets of dried sputum may be infectious in the AIR for ________
8-10 days
Cultures may be killed from sunlight within ________
2 hrs
Boiling easily kills mycobacteria for _______ minutes
10 minutes
Act as Digestant
NALC
Act as decontaminant
NaOH
Pathognomonic for MTB
Laghan’s cells
Ghon complexes (chest X-ray)
MTB colonies
Cauliflower like appearance
Number of spx. submitted for MTB detection
2 specimens
* 1st - Early morning
* 2nd - Random
Source: DOH
Ideal size of the AFB smear
2x3 cm
Use to diagnose latent TB infection
IGRAS - Interferon gammar release Assay
Preffered stain for Tissue
Kinyou’s (Cold method)
Preffered stain for M. laprae
Fite faraco
Before reporting MTB negative, ________ fields should be examined
300 fields
Inhibitor of Egg based media for MTB
Malachite green
MTB in Pappenheim’s stain
Red
MTB in Baumgartin’s stain
Blue
Type I: Phytochromogens
“MASK”
Pigmentin LIGHT tube
* M. marinum
* M. asiaticum
* M. semiae
* M. kansasii
Type II: Schtochromogen
“XS sci GORDON”
Produce pigment in DARK and LIGHT tube
* M. xenopi
* M. szaulgai
* M. scrofula
* M. gordonae
Type IV: Rapid grower
“Sir Felix Cute na Pogi
Grows fewer than 7 days (5-7)
* M. smegmatis
* M. fortuitum
* M. cheloniae
* M. phlei
Yellow bacillus
M. kansasi
Swimming pool granulomatous
M. marinum
Tap water bacillus
M. gordonae
TB in animals and used in MTB vaccine
M. bovis
