MT4 - Brucella Flashcards
Rose-Bengal test is used for the detection of antibodies against brucellae
T
Slide agglutination test (Rose-Bengal test)
Brucella species cannot tolerate high temperature (60 °C) and low pH (pH: 4).
T
Brucellae can be detected by staining in the placenta of aborted foetuses in the case of brucellosis.
T
Köster-staining (MZN), Brucella: red, B. ovis & B. canis NO = blue
Humans can acquire brucellosis from raw milk.
T
raw milk or dairy products from unpasteurized milk: infected animals.
Europe is free from Brucella ovis
F
Undulant fever, malaise, inappetence, fatigue, and muscle and joint pains can be the clinical sings of human brucellosis.
T
Brucella species are generally zoonotic agents
T
- B. melitensis
- B. abortus
- B.suis
- B.canis
B. ovis = NOT ZOONOTIC
Brucella are facultative IC agents.
T
Facultative intracellular pathogens
Brucella is generally a notifiable disease
T
Among Brucella species Brucella melitensis is the most pathogenic species for humans.
T
- B. melitensis
- B. abortus
- B.suis
- B.canis
More than one serological test must be carried out from one serum sample at the same time in the case of serodiagnostics of brucellosis
T
Brucellosis is generally a notifiable disease.
T
Brucellosis is a chronic disease:
T
Brucella species are gram-negative, fastidious, coccoid rod-shaped bacteria
T
There is a serological relationship among some brucella species, because of the same polysaccharides in the cell wall
T
Worldwide distribution is characteristic for brucellosis
T
Köster staining is used for staining of brucellae
T
Brucella can be both acute or chronic in human
T
Brucella melitensis is endemic in Mediterranean area
T
Endemic :Middle-East, Central Asia, Latin America, Mediterranean Region
Brucella species are widely distributed all over the world.
T
Brucella species are serologically uniform
F
variations in antigenic structures among different Brucella species.
Brucella species need cysteine or cystine as an additive for the culture
F - Tularemia needs
Brucella species are not zoonotic agents.
F
B.ovis: not zoonotic
Susceptibility of humans to different Brucella species is different.
T
Consumption of raw milk is an important route of human Brucella infection
T
Brucella need chocolate agar to culture.
F
There is a close antigenic relationship between B. ovis and B. melitensis.
F
B. suis is a zoonotic agent.
T
Brucella ovis needs CO2 to culture.
T
Brucella are Gram-positive and Koster-positive bacteria
F
Gram - , koster + bacteria
The antigens of Brucella are uniform.
F
The majority of the Brucella species can infect humans.
T
Brucella are not fastidious bacteria, they can be cultured on simple nutrient agar
F
Brucella species are non-motile, Gram-negative, small coccoid rods
T
There is a serological relationship among B. abortus, B. melitensis and B. ovis, because of the same surface polysaccharides
F
There are no brucellosis-free countries in the world.
F
Free: Canada, Japan, Australia, New-Zealand, Israel
EU countries: most of them free
Most of the Brucella species are zoonotic agents.
T
There is close antigenic relationship between Brucella bovis and Brucella ovis.
F
Brucella suis is a zoonotic agent.
T
Brucella canis is stained red as a result of Koster-staining.
F
B. ovis & B. canis NO
Brucella species are not fastidious bacteria, we can use nutrient agar in diagnostic work.
F
fastidious bacteria = specific nutritional requirements for growth
There is a strong relationship in antigenic structure of Brucella canis and Brucella ovis.
T-A
Fetus, fetal membrane and blood sample from the dam must be send to the diagnostic institute in abortion case.
T
fetus, fetal membranes (placenta), and blood samples from the dam
Human brucellosis was described for the first time by David Bruce in Malta Island in 1886
T
Brucella abortus stains red as a result of Koster-staining.
T
B. ovis & B. canis no
There is a strong relationship in the antigenic structure of B. melitensis and B. suis
T
All of the Brucella species are zoonotic agents.
F
B.ovis is not
Vaccination is a regularly used prevention method in the brucellosis free countries of EU
F
In the case of a same serum sample it is advisable to carry out more serological tests for the detection of antibodies against Brucella species, because different serological tests can show
different results
T
Rev I. is an inactivated vaccine strain.
F
live vaccine
There are no differences in serological features of Brucella species with veterinary importance.
F
Brucella are facultative intracellular bacteria
T
With Koster-staining all Brucella are positive.
F-A
Sheep and goat are the reservoirs for Brucella
F
Hungary is Brucella free.
F-A
Humans are resistant to Brucella melitensis.
F
Raw milk consumption can be the source of human brucellosis
T
Vaccination against brucellosis is common in the EU
T-A but im unsure
Attenuated vaccine strains can cause abortion in pregnant animals
T
Focal necrosis and inflammation are seen in fetuses aborted due to Brucella
T
Brucella is an obligate pathogen.
T
Brucella are a notifiable disease.
T
Brucella can be found on the mucous membranes of the genital tract.
T
All Brucella can be stained with Koster staining.
F
There will be cross-reactions between S-type colonies in case of Brucella
T
Brucellosis is found in most parts of the world.
T
In sexually immature animals, Brucella multiplies in the lymph nodes.
T
Brucellosis is usually introduced by infected asymptomatic animals.
T
Abortion caused by brucellosis is followed by retained fetal membranes.
T
An attenuated vaccine is used in Hungary for prevention of B. melitensis.
F
not in eu - mostly free
Brucella has average resistance
T
Brucella is seen only in the northern hemisphere.
F
Brucella species can propagate in the soil
F- but cant see in pq file
