MT 1 random Flashcards
Coccidiosis based on location in intestines
Duodenum: -E.acervulina (chicken) -E.intestinalis, E.magna, E.irresidua (rabbit) -(+ I.felis, I.rivolta, I.canis, I.ohioensis, I.burrowsi) Jejunum: -E.acervulina, E.brunetti, E.necatrix, E.maxima -(+ E.anseris, E.nocens - geese) -E.intestinalis, E.magna, E.irresidua -(+ I.suis, I.felis, I.rivolta, I.canis, I.ohioensis, I.burrowsi) Ileum: -E.brunetti, E.necatrix -(+ E.anseris, E.nocens) -E.intestinalis, E.magna, E.irresidua -E.bovis, E.zuernii (cattle) -(+ I.suis, I.felis, I.rivolta, I.canis, I.ohioensis, I.burrowsi) Caecum: -E.brunetti, E.tenella -E.flavescens, E.piriformis (rabbit) -E.bovis, E.zuernii Colon: -E.brunetti -E.bovis, E.zuernii Rectum: -E.brunetti Cloaca: -E.brunetti
Intestines - how to know which part?
- Duodenum: can see the pancreas
- Villi: only in lumen of small intestines
- Birds: have 2x caecum
Coccidiosis species
- Chicken: E.tenella, E.necatrix, E.brunetti, E.maxima, E.acervulina, E.mitis, E.praecox
- Geese: Int. form: E.anseris, E.nocens or renal form: E.truncata
- Cattle: E.bovis, E.zuernii
- Rabbit: Biliary form - E.stiedai or Int. form - E.intestinalis, E.magna, E.irresidua, E.flavescens, E.piriformis
- Pig: I.suis
- Cats: I.felis, I.rivolta
- Dog: I.canis, I.ohioensis, I.burrowsi
- Sheep: E.bakuensis
Parasites found with flotation method
- Giardia duodenalis
- Entamoeba histolytica
- Coccidiosis of chicken - Eimeria
- Coccidiosis of geese - Eimeria (int. and renal form)
- Coccidiosis of cattle - Eimeria (not enough)
- Coccidiosis of rabbit - Eimeria (biliary and int. form) (not enough)
- Coccidiosis of pig - Eimeria and Isospora suis
- Coccidiosis of Ca - Isospora
- Cryptosporidium
- Toxoplasma gondii (cat faeces only)
- Sarcocystiosis - intestinal sarcosporidiosis
Parasites found with feacal smear
- Giardia duodenalis
- Entamoeba histolytica
- Coccidiosis of pig - Eimeria and Isospora suis
- Cryptosporidium
Oocytes characteristics of different parasites
- Coccidiosis - Eimeria: can be sporulated or unsporulated. Sporulated oocyte contain 4 sporocysts each with 2 banana-shaped sporozoites. Has a polar cap. Unsporulated contains a zygote within the shell.
- Coccidiosis - Isospora: sporulated oocyst contains 2 sporocyst with four sporozoites each (Ø cap)
- I.suis: spherical
- I.felis: oval
- Cryptosporidium: spherical/elliptical w. obscure internal structure (four sporozoites) (Kinyoun staining)
- Toxoplasma gondii: sporulated oocysts contains 2 sporocysts with 4 sporozoites each (Ø cap - sim. to Isospora) - indistinguishable from Hammondia Hammondi.
- Sarcocystiosis: sporulated oocysts contains two sporocysts with 4 sporozoites each
Necessary for sporulation
- Temp.
- O2
- Water
Staining types and parasites their used for
- Giemsa: pink/purple/blue (Trypanosomas, Leishmania, Giardia, Trichomonas, Cryptosporidium, Babesia, Theileria)
- Kinyon: blue - parasites are red/white (Cryptosporidium,
- PAS: pink/purple/blue (Histomonas, Encephalitozoa)
- Zeehle-Neelsen: blue - parasites pink/purple (Cryptosporidium, Encephalitozoa)
- Safranin-methylene: safranin - red, MB - blue (Cryptosporidium)
- Gram: pink/purple/blue (Histomonas: G-, Encephalitozoa: G+)
- HE stain: pink/purple/blue (Sarcocystiosis)
- Lugol: yellow/brown (Giardia, Entamoeba)
Parasites found in kidney
- Histomonas: necrotic lesions
- Coccidiosis of geese - Eimeria truncata: enlarged and cut surface with greyish-white foci
- Cryptosporidiosis in birds: rarely histological changes
- Sarcocystiosis: necrotic lesions, groups of merozoites or small schizonts in smears
- Babesiosis: degeneration
- Encephalitozoon: 2-4 mm large greyish dents on the surface (rabbits and dogs)
Parasites found in blood
-Trypanosoma dourine (peripheral blood sample/motility/microscopic exam. bw. BC and P/smear w. Giemsa)
-Trypanosoma nagana (peripheral blood sample/motility/microscopic exam. bw. BC and P)
-Trypanosoma surra (smear w. Giemsa)
-Hepatozoon canis (elliptic gamonts in neutrophilic granulocytes/monocytes)
-Babesiosis (piroplasms in RBC´s - Giemsa, red nucleus,
blue cytoplasm)
-Theileria (merozoites in RBC´s - Giemsa)
Parasites found in liver
- Leishmania: enlarged, biopsy
- Trichomonas of birds: necrotic lesions in hepatic and generalized forms
- Histomonas: deep, circular, crateriform necrotic foci (0.5-2.0 cm) w. sharp edges, dry cut surface, generalized form in guinea fowl: wet cut surface of liver
- Coccidiosis of rabbit - Eimeria stiedai (biliary form): hepatomegaly, greyish yellow nodules
- Hepatozoon canis: inflammatory infiltrates, necroses
- Toxoplasmosis: necrotic foci and granulomas (rabbits)
- Sarcocystiosis: necrotic lesions
- Babesiosis: degeneration
Parasites found in intestines
- Giardia duodenalis
- Histomonas meleagridis (ceacum)
- Entamoeba histolytica
- Coccidiosis of chicken - Eimeria
- Coccidiosis of geese - Eimeria
- Coccidiosis of cattle - Eimeria
- Coccidiosis of pig - Eimeria and Isospora suis
- Coccidiosis of Ca - Isospora
- Cryptosporidium of mammals+birds
- Sarcocystiosis - intestinal sarcosporidiosis
Parasites found in skin scrapings/biopsy/tissue culture
- Leishmania - skin scrapings
- Besnoitia besnoiti - skin biopsy
- Toxoplasma gondii (tissue culture)
Parasites found in biopsy
- Leishmania (from lnn/liver/spleen/BM/skin - Giemsa)
- Theileria (ln - Giemsa)
- Toxoplasmosis (stained or by IFAT)
- Besnoitia besnoiti (skin)
Parasites found in genitals
- Trypanosoma equiperdum (scraping of muc.mem. in fresh cases)
- Trichomonas foetus (washing preputium w. phys. saline or special sol; collecting of vaginal/uteral discharge; seminal fluids)
- Besnoitia besnoiti (cysts)
Parasites found in respiratory tract
- Besnoitia besnoiti (cysts)
- Cryptosporidium of birds
Parasites found in oseophagus
- Trichomonas gallinae (also in crop)
- Sarcocystiosis (cysts)
Parasites found in skin lesions
-Histomonas meleagridis
Parasites found with oocyst counting (OPG)
- Coccidiosis of chicken - Eimeria - Mc.Master method
- Coccidiosis of cattle - Eimeria - Mc.Master method
- Coccidiosis of rabbit - Eimeria (int. form)
Parasites found in eye
-Besnoitia besnoiti (cysts in sclera, conjunctiva)
Parasites found in heart
-Sarcocystiosis (cysts)
Parasites found in muscles
-Sarcocystiosis - muscle sarcosporidiosis
Parasites found in urine
-Encephalitozoon cuniculi
Important for Cryptosporidiosis
- Autoinfection
- IC but extracytoplasmic
- Kinyoun staining: red (thick wall) and white (thin wall) are the oocysts, with blue background (good for small parasites).
Coccidiosis life cycle
- Oocyst swallowed by animal and goes into gut.
- O. release sporocysts which release sporozoites which invade gut cells and build Scizonts w. trophozoites inside –> Schizogony/Merogony
- Schizonts release MZ which infect new gut cells.
- MZ Build macrogamonts (female) and microgamonts (male) sperms - Miro goes over to Macro –> Gametogony
- Grows zygot and black cell nucleus
- Zygot becomes oocyst. Younger oocyst builds wall (protein).
- Free unsporulated oocyst is shredded in faeces.
- ..
Form of parasites we detect in different species
- Trypanosomas: trypomastigotes - motile trypanosomas in blood
- Leishmania: amastigote in smears/scrapings
- Giardia: trophozoites (vegetative form) - pear shaped, symmetrical, two nuclei, two axostyles and 4 pairs of flagella, or cysts (infectious form)
- Trichomonas foetus: trophozoites - pear shaped, one flagella forming membrane backwards, 3 flagella forwards
- Trichomonas gallinae: trophozoites - pear shaped, one flagella forming membrane backwards, 4 flagella forwards
- Histomonas: flagellate form (1 flagella) in caecal lumen, ameboid (Ø flagella) form in tissues - pleomorphic
- Entamoeba: trophozoites (vegetative form) - irregular shape and vesicle-like nucleus or cysts (infectious form) - round with 4 nuclei
- Eimeria: unsporulated and sporulated oocysts, schizonts (macroschizont or globidium) or merozoites - long, crescent shaped (inside schizont)
- Isospora: unsporulated and sporulated oocysts
- Cryptosporidiosis, Toxoplasmosis, Sarcocystiosis: oocysts
- Besnoitia: cysts in sclera, conjuctiva, RT and genitals
- Hepatozoon: elliptic gamonts in monocytes (sausage like)
- Babesiosis: piroplasms in blood
- Theileria: schizonts in biopsy of ln., Koch´s bodies in lymphyctes or merozoites - rod-shaped and blue, round, oval and ring-shaped forms, in blood.
- Encephalitozoon: spores in urine of rabbits
Parasites in carnivores
- Trypanosoma brucei + congolense (nagana) + brucei evansi (surra)
- Leishmania (+hu)
- Giardia (mainly dogs, +mammals +birds)
- Entamoeba histolytica (+Hu, monkey, kitten, young dog)
- Coccidiosis - I.felis, I.rivolta, I.canis, I.ohioensis, I.burrowsi
- Hepatozoon canis (only)
- Sarcocystiosis (final host)
- Toxoplasmosis gondii (final h. felinae, im. mammals+birds)
- Babesia canis + vogeli + gibsoni
Parasites in birds
- Histomonas meleagridis (only)
- Trichomonas gallinae (only)
- Cryptosporidium baileyi + meleagridis (only)
- Coccidiosis - Eimeria…
Parasites in horses
- Trypanosoma equiperdum (dourine) + brucei brucei (nagana) + brucei evansi (surra) + equinum
- Sarcosporidia equicanis (im. host)
- Babesia caballi
- Theileria equi
Parasites in cattle
- Trypanosoma vivax (nagana) + brucei evansi (surra)
- Besnoitia besnoiti (im. host)
- Trichomonas foetus (only)
- Sarcosporidia hirsuta (im. host)
- Cryptosporidium parvum + muris (many mammals, esp. young calves)
- Coccidiosis - E.zuernii + bovis
- Babesia divergens + bovis + bigemina + major
- Theileria annulata (Ru)
Parasites in sheep
- Sarcosporidia ovicanis + gigantea (im. host)
- Theileria annulata (Ru)
Parasites in pigs
-Coccidiosis - I.suis + Eimeria
Parasites in rabbits
- Coccidiosis - E.stiedai + inestinal eimeria
- Encephalitozoon cuniculi (mammals, mainly rabbits)
Histological findings of Coccidiosis of chickens
- E.acervulina: duodenum+jejunum, visible white foci or transverse bands - “ladder like”, content watery, mucus w/ø blood → not really dangerous
- E.maxima: mid-small int. Petechia, with blood (more dangerous) on surface of int. mucosa, infected area = “salt and pepper like”
- E.necatrix: jejunum+ileum, haemorrhagic, thickened, swollen, unclotted blood in lumen, scattered white spots - “pepper and salt like”
- E.tenella: caecum, enlarged, extensive haemorrhages, unclotted/partially/clotted blood in lumen, deep erosions of epithelium, long-term: caseous material in lumen adhere to mucosa
- E.brunetti: low small int., prox. caecum, colon, rectum, cloaca, haemorrhagic, catarrhal exudate and coag. necrosis, sometimes petechia on tip of villi in rectum - “-ladder like”
Parasites using serological methods
- Trypanosoma Dourine + Nagana
- Leishmania
- Trichomonas foetus
- Besnoitia besnoiti
- Babesia
- Theileria
Parasites using immunological methods
- Giardia
- Trichomonas foetus
- Entamoeba
- Cryptosporidiosis
- Toxoplasmosis (Sabin Feldman dye test, Complement Fixation Test, ELISA, IHAT, IFAT)
- Encephalitozoon (IFAT, CIA, IIR)
Parasites using PCR technique
- Leishmania
- Cryptosporidiosis (molecular techniques)
- Toxoplasmosis (human CSF)
Distinktiv clinical sign with parasites
- Babesia: icterus
- Trypanosoma - dourine: dollar spots
- T. - Nagana: odeama of limbs and genitals
- T. - Equinum: paralysis of HL and hips
- Cryptosporidium mammals: v. bloody diarrhea, young calves
- Cryptosporidium birds: signs of resp. dis.
- Coccidia: watery diarrhoea
- Histomonas: black come
- Leishmania: creeping eruption
- Theileria: swelling of lnn., dyspnoea, tachycardia, petechial hemorrhages on vulva, under tongue