Lab 6 - Hepatozoonosis of dog, Babesiosis, Theileriosis, Encephalitozoonosis Flashcards
What size are the Encephalitozoa spores?
1-2µm
Which staining is used for Encephalitozoa?
Gram +
Which cells does Babesia first infect?
RBC
Koch´s bodies are…?
Schizonts in white blood cells
B. vogeli can infect?
Dogs
What is the size of the Koch´s bodies?
10-12 micrometers.
Spores of E. cuniculi can be detected in the …… of rabbits?
Urine
During necropsy thickened and nodular vessels in arteries of viscera can be seen with the naked eye in?
Encephalitozoonosis
T. annulata is seen in?
Cattle
In encephalitozoonosis eye disorders are seen in?
Fox
What type of animals do Babeosis affect?
Mammals
Which species does not have haemoglobinuria as a clinical sign of babeosis?
Horses
What is the difference between babesia and Therilria?
C. Theileria can develop in lymphocytes and in RBC - babesia can only develop in RBC
In which specie may abortion occur in Babesiosis?
Cattle
What is the species of babesia that have zoonotic character amongs following cases?
B. Bigemina (in notes it says B. divengens but it is rare and Bigemina is more pathogenic)
Which vertebrate animals are susceptible to encephaliozoonosis?
Many mammals (Rabbits, Rodents, Carnivores, Primates)
Thileria develops where first?
Lymphocytes in the Lymph nodes
In what cells do the Koch bodies develop?
a. Lymphocytes
b. Neutrophils
c. Monocytes
d. Erythrocytes
a. Lymphocytes
What is the symptom that doesn´t belong to the common symptoms of Babesiosis of the dog?
a. Jaundice
b. Fever
c. Hemoglobinuria
d. Abortion
d. Abortion
What is the difference between the cell preference of Babesia and Theleria?
a. Babesia prefers RBC, Theleria does not
b. Theleria prefers RBC, Babesia does not
c. Theleria can develop in Lymphocytes and Babesia does not
d. Babesia can develop in Lymphocytes and Theleria does not
c. Theleria can develop in Lymphocytes and Babesia does not
Which babesia has zoonotic significance?
B. divergans of the Cow
Form of Thileria that occurs in RBC?
Piroplasm
In which cells do gamonts of hepatozoon develop?
Neutrophil granulocytes (monocytes) (blood smear)
Correct term for host range of hepatozoonosis?
Obligate Heteroxenous
Animal that aborts fetus in babesiosis?
Cow
Symptom not in dog infected with babesiosis?
Abortion
Which is the host that may show clinical signs in the eye during babesia infection?
The Dog (they get keratitis and iridocyclitis when infected with B. Canis, Vogeli or Gibsoni)
What size are the Encephalitozoon spores?
1.5x2.5µm elleiptic or oval.
In encephalitozoonosis eye disorders are seen in which species?
Fox and dog (keratitis and night blindness)
Which staining can you not use in detection of encephalitozoa?
He staining (Can use PAS, Gram+, Ziehl Neelson and indian ink for the spores)
Causative agent of Hepatozoonosis?
Hepatozoon canis - wild carnivores
Biological vector of Hepatozoonosis?
Brown dog tick (Rhipicephalus sanguineus, transstadial transmission)
Necropsy findins in Hepatozoonosis?
- All infected organs: inflammatory infiltrates, necroses
- Schizonts 50-100 microm., nuclei in the periphery
Which species of Babesiosis can infect horses?
B.caballi
Which species of Babesiosis can infect cattle?
B.divergens*, B.bovis, B.bigemina, B.major
Which species of Babesiosis can infect dog?
B.canis, B.vogeli, B.gibsoni
Vector of Babesiosis?
Ticks
What can we detect in RBC of Babesia?
Piroplasms
Staining used for Babesia blood film?
Giemsa
What can we detect as paired near edge of cells in Babesia?
“small babesia”
What can we detect as paired at acute angle of RBC in Babesia?
“large babesia”
Necroscopy findings in Babesia?
- anaemia, jaundice, splenomegaly
- sub-epicardial and sub-endocardial haemorrhages
- red and brown-red urine in bladder
- degeneration of liver and kidneys
- catarrh with petechiae in gastro-intestinal mucosa
Which animals are infected by Theileriosis?
Horses, Ruminants in Southern Europe, Africa, Asia
Species of Theileriosis?
T.equi, T.annulata
What can be detected in biopsy samples of lymph nodes infected with Theileriosis?
Shizonts
Staining method of Theileriosis?
Giemsa
What can be detected in red cells of blood films stained with Giemsa infected by Theileriosis?
Merozoites (0.5-2.7 μm, rod-shaped and blue; round, oval and ring-shaped forms)
Necroscopy findings of Theileriosis?
- haemorrhages in serous and mucous membranes
- swelling of lymph nodes and spleen
- ulcers in abomasum and intestine, pulmonary oedema, cachexia – in chronic cases
Species of Encephalitozoonosis that can infect mammals?
Encephalitozoon cuniculi
Necroscopy findings of Encephalitozoon?
- macroscopic, 2-4 mm large greyish dents on the surface of the kidney of rabbits and dogs
- histology of kidney: chronic interstitial nephritis
- histology of brain: multifocal granulomatous encephalitis - perivascular infiltration by
lymphocytes, necrotic foci surrounded by macrophages, lymphocytes containing
developing stages of parasite - encephalitozoa are Gr+ and PAS+, Ziehl-Neelsen+
- thickened and nodular vessels in arteries of viscera, especially in superficial coronary
arteries which can be seen by naked eyes
