Lab 6 - Hepatozoonosis of dog, Babesiosis, Theileriosis, Encephalitozoonosis Flashcards

1
Q

What size are the Encephalitozoa spores?

A

1-2µm

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2
Q

Which staining is used for Encephalitozoa?

A

Gram +

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3
Q

Which cells does Babesia first infect?

A

RBC

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4
Q

Koch´s bodies are…?

A

Schizonts in white blood cells

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5
Q

B. vogeli can infect?

A

Dogs

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6
Q

What is the size of the Koch´s bodies?

A

10-12 micrometers.

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7
Q

Spores of E. cuniculi can be detected in the …… of rabbits?

A

Urine

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8
Q

During necropsy thickened and nodular vessels in arteries of viscera can be seen with the naked eye in?

A

Encephalitozoonosis

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9
Q

T. annulata is seen in?

A

Cattle

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10
Q

In encephalitozoonosis eye disorders are seen in?

A

Fox

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11
Q

What type of animals do Babeosis affect?

A

Mammals

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12
Q

Which species does not have haemoglobinuria as a clinical sign of babeosis?

A

Horses

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13
Q

What is the difference between babesia and Therilria?

A

C. Theileria can develop in lymphocytes and in RBC - babesia can only develop in RBC

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14
Q

In which specie may abortion occur in Babesiosis?

A

Cattle

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15
Q

What is the species of babesia that have zoonotic character amongs following cases?

A

B. Bigemina (in notes it says B. divengens but it is rare and Bigemina is more pathogenic)

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16
Q

Which vertebrate animals are susceptible to encephaliozoonosis?

A

Many mammals (Rabbits, Rodents, Carnivores, Primates)

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17
Q

Thileria develops where first?

A

Lymphocytes in the Lymph nodes

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18
Q

In what cells do the Koch bodies develop?

a. Lymphocytes
b. Neutrophils
c. Monocytes
d. Erythrocytes

A

a. Lymphocytes

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19
Q

What is the symptom that doesn´t belong to the common symptoms of Babesiosis of the dog?

a. Jaundice
b. Fever
c. Hemoglobinuria
d. Abortion

A

d. Abortion

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20
Q

What is the difference between the cell preference of Babesia and Theleria?

a. Babesia prefers RBC, Theleria does not
b. Theleria prefers RBC, Babesia does not
c. Theleria can develop in Lymphocytes and Babesia does not
d. Babesia can develop in Lymphocytes and Theleria does not

A

c. Theleria can develop in Lymphocytes and Babesia does not

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21
Q

Which babesia has zoonotic significance?

A

B. divergans of the Cow

22
Q

Form of Thileria that occurs in RBC?

A

Piroplasm

23
Q

In which cells do gamonts of hepatozoon develop?

A

Neutrophil granulocytes (monocytes) (blood smear)

24
Q

Correct term for host range of hepatozoonosis?

A

Obligate Heteroxenous

25
Q

Animal that aborts fetus in babesiosis?

A

Cow

26
Q

Symptom not in dog infected with babesiosis?

A

Abortion

27
Q

Which is the host that may show clinical signs in the eye during babesia infection?

A

The Dog (they get keratitis and iridocyclitis when infected with B. Canis, Vogeli or Gibsoni)

28
Q

What size are the Encephalitozoon spores?

A

1.5x2.5µm elleiptic or oval.

29
Q

In encephalitozoonosis eye disorders are seen in which species?

A

Fox and dog (keratitis and night blindness)

30
Q

Which staining can you not use in detection of encephalitozoa?

A

He staining (Can use PAS, Gram+, Ziehl Neelson and indian ink for the spores)

31
Q

Causative agent of Hepatozoonosis?

A

Hepatozoon canis - wild carnivores

32
Q

Biological vector of Hepatozoonosis?

A

Brown dog tick (Rhipicephalus sanguineus, transstadial transmission)

33
Q

Necropsy findins in Hepatozoonosis?

A
  • All infected organs: inflammatory infiltrates, necroses

- Schizonts 50-100 microm., nuclei in the periphery

34
Q

Which species of Babesiosis can infect horses?

A

B.caballi

35
Q

Which species of Babesiosis can infect cattle?

A

B.divergens*, B.bovis, B.bigemina, B.major

36
Q

Which species of Babesiosis can infect dog?

A

B.canis, B.vogeli, B.gibsoni

37
Q

Vector of Babesiosis?

A

Ticks

38
Q

What can we detect in RBC of Babesia?

A

Piroplasms

39
Q

Staining used for Babesia blood film?

A

Giemsa

40
Q

What can we detect as paired near edge of cells in Babesia?

A

“small babesia”

41
Q

What can we detect as paired at acute angle of RBC in Babesia?

A

“large babesia”

42
Q

Necroscopy findings in Babesia?

A
  • anaemia, jaundice, splenomegaly
  • sub-epicardial and sub-endocardial haemorrhages
  • red and brown-red urine in bladder
  • degeneration of liver and kidneys
  • catarrh with petechiae in gastro-intestinal mucosa
43
Q

Which animals are infected by Theileriosis?

A

Horses, Ruminants in Southern Europe, Africa, Asia

44
Q

Species of Theileriosis?

A

T.equi, T.annulata

45
Q

What can be detected in biopsy samples of lymph nodes infected with Theileriosis?

A

Shizonts

46
Q

Staining method of Theileriosis?

A

Giemsa

47
Q

What can be detected in red cells of blood films stained with Giemsa infected by Theileriosis?

A

Merozoites (0.5-2.7 μm, rod-shaped and blue; round, oval and ring-shaped forms)

48
Q

Necroscopy findings of Theileriosis?

A
  • haemorrhages in serous and mucous membranes
  • swelling of lymph nodes and spleen
  • ulcers in abomasum and intestine, pulmonary oedema, cachexia – in chronic cases
49
Q

Species of Encephalitozoonosis that can infect mammals?

A

Encephalitozoon cuniculi

50
Q

Necroscopy findings of Encephalitozoon?

A
  • macroscopic, 2-4 mm large greyish dents on the surface of the kidney of rabbits and dogs
  • histology of kidney: chronic interstitial nephritis
  • histology of brain: multifocal granulomatous encephalitis - perivascular infiltration by
    lymphocytes, necrotic foci surrounded by macrophages, lymphocytes containing
    developing stages of parasite
  • encephalitozoa are Gr+ and PAS+, Ziehl-Neelsen+
  • thickened and nodular vessels in arteries of viscera, especially in superficial coronary
    arteries which can be seen by naked eyes