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Histopathology > Mounting > Flashcards

Mounting Flashcards

1
Q

Refractive index of glass

A

1.518

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2
Q

Two most commonly used mounting media

A

Eukitt
Entellan

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3
Q

Enumerate the water soluble mountants (aqeous)

A

Water
Glycerin
Farrant’s medium
Apathy’s medium
Brun’s fluid

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4
Q

Low refractive index
Good only for temporary mounting

A

Water

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5
Q

Also used as a preservative
Has high index of refraction

A

Glycerin (RI: 1.47)

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6
Q

Does not solidify upon storage; RI: 1.43

A

Farrant’s medium (gum syrrup)

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7
Q

Used for methylene blue-stained nerve preparations and as general purpose aqueous mountant

A

Apathy’s medium

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8
Q

Recommended for mounting frozen sections from water or paraffin sections which require dehydration and clearing

A

Brun’s fluid

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9
Q

Enumerate resinous mountants

A

Canada Balsam (1.524)
DPX (1.532)
XAM (1.52)
Clarite (1.544)

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10
Q

This mountant is used for preparations that have been dehydrated and cleared in xylene or toluene and are recommended for majority of staining methods

A

Resinous mountants

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11
Q

Natural resin extracted from the Canadian Tree (Abus balsamea)

refractive index: ___

A

Canada balsam (1.524)

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12
Q

Recommended for whole mounts and for thick sections because it does not shrink much

A

Canada balsam (1.524)

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13
Q

Canada balsam is miscible with ___

A

Xylene

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14
Q

Recommended for staining small tissue sections

A

DPX (1.532)

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15
Q

Synthetic resin mixture in xylene in pale yellow or colorless solution

A

XAM (1.52)

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16
Q

Synthetic resin which is soluble in xylene (usually diluted with 60% xylene)

A

Clarite (1.544)

17
Q

This is the process of sealing the margins of the cover slip to prevent escape of fluid or semi-fluid mounts and evaporation of mountant to immobilize the coverslip and to prevent sticking of slides upon storage

A

Ringing

18
Q

Components of Kronig cement

A

2 parts paraffin wax
4-9 parts powdered colophonium resin (heated and filtered)

Histopathology (16 decks)

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