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Histopathology > Lec Staining > Flashcards

Lec Staining Flashcards

1
Q

Give the specific stains and what it stains

A

Perl’s Prussian blue- hemosiderin (hemoglobin)
Periodic Acid Schiff- polysaccharides (carbohydrates)

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2
Q

Give the stains in direct/simple staining

A

Methylene blue
Eosin

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3
Q

Examples of indirect staining

A

Mordant
Accentuator

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4
Q

Examples of mordant

A

Potassium alum with hematoxylin in Ehrlich’s hematoxylin
Iron in weigert’s hematoxylin

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5
Q

Examples of accentuators (2)

A

KOH in Loeffler’s methylene blue
Phenol in carbon thionine and carbol fuchsin

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6
Q

Stains used in progressive staining

A

Gill’s haematoxylin
Mayer’s haematoxylin

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7
Q

Stains used in regressive staining

A

Harris’ haematoxylin
Delafield’s haematoxylin

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8
Q

This staining does not stain nucleus

A

Vital staining

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9
Q

mixture of PICRIC ACID and ACID FUCHSIN for the demonstration of CT, MUCIN, and ELASTIC TISSUE

A

Van Gieson’s (Acid Fuschin PIcric Acid)

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10
Q

basic acridine flurochrome which permits discrimination between DEAD AND LIVING cells, giving GREEN fluorescence for DNA

A

Acridine Orange

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11
Q

for CALCIUM SALTS and PHOSPHATASE ACTIVITY

A

Acridine Red 3B

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12
Q

CYTOPLASMIC STAIN used for COUNTERSTAINING of EPITHELIAL CELLS

A

Aniline Blue

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13
Q

stain ACID MUCOPOLYSACCHARIDES, more specific for CT and EPITHELIAL MUCIN

A

Alcian Blue

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14
Q

a PLASMA STAIN utilized for deep staining of acid fast organisms’ MITOCHONDRIA, and a differentiation of SMOOTH MUSCLES with the use of PICRIC ACID

A

Basic Fuchsin
(Carbol fuchsin, Coleman’s Feulgen, Schiff’s, Mallory’s fuchsin, Aldehyde fuchsin/Gomori’s)

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15
Q

staining HAEMOGLOBIN

A

Benzidine

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16
Q

stains DIPTHERIA ; used for contrast stain for Gram’s technique, acid-fast, and Papanicolaou method

A

Bismarck Brown

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17
Q

used as a CHROMATIN stain for fresh materials in smear preparations

A

Carmine

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18
Q

combined with ALUMINUM CHLORIDE to stain GLYCOGEN

A

Best Carmine

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19
Q

a mordanted dye acting as a basic dye and staining ACID SUBSTANCES

A

Mayer’s Carmalum solution

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20
Q

recommended for staining of FIXED SECTIONS

A

Celestine Blue

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21
Q

best known as INDICATOR; may be used as a stain for axis cylinders in EMBRYOS; used for staining ELASTIC TISSUES, AMYLOID, and MYELIN (Krajian’s Method)

A

Congo Red

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22
Q

a NUCLEAR or CHROMATIN stain used for staining AMYLOID in FROZEN SECTIONS and PLATELETS

A

Crsytal Violet

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23
Q

mixture of CV, Methyl Violet, and Dextrin

A

Gentian Violet

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24
Q

stain blood to DIFFERENTIATE LEUKOCYTES

25
Q

used for METALLIC IMPREGNATION, made up of gold chloride and mercuric chloride

A

Gold Sublimate

26
Q

the OLDEST and stains AMYLOID, CELLULOSE, STARCH, CAROTENES, and GLYCOGEN

A

Gram’s Iodine and Lugol’s Iodine

27
Q

used for staining MITOCHONDRIA

A

Janus Green B

28
Q

contrast stain for ASCARIS EGGS and ERYTHROCYTES, and BACTERIAL SPORE stain

A

Malachite Green

29
Q

stains CHROMATIN GREEN when an acid is present

A

Methyl Green

30
Q

DIPTHERIA diagnosis

A

Methylene Blue

31
Q

stains PLASMA CELLS, fresh SPUTUM for malignant cells, evaluation and differentiation of bacterial organisms

A

Methylene Blue

32
Q

coloring NUCLEI of LEUKOCYTES REDDISH-PURPLE in presence of METHYLENE BLUE

A

Methylene Violet

33
Q

for observing CELL GRANULES and VACUOLES of phagocytic cells

A

Neutral Red

34
Q

substitute for carbol fuchsin in acid-fast staining

A

Night Blue

35
Q

an excellent stain for ELASTIC FIBERS; demonstrates the finest and most delicate fibers in skin used for DERMATOLOGIC STUDIES

36
Q

commonly used as a fixative and is used to stain FAT

A

Osmium Tetroxide

37
Q

utilized for manufacture of PAINTS
used as a MICROANATOMICAL contrast stain for demonstration of the circulatory system by injection (intravital staining)

A

Prussian Blue

38
Q

used with osmic acid to fix and stain BLOOD and GLANDULAR TISSUES

A

Rhodamine B

39
Q

used in identification of SPIROCHETES, RETICULUM, and OTHER FIBER STAINS

A

Silver Nitrate

40
Q

stains NISSL granules or CHROMOPHILIC BODIES and nuclear stain for FIXED TISSUES

A

Toluidine Blue

41
Q

used as a substitute for thionine in fresh tissue sections

A

Toluidine Blue

42
Q

stains NEUROGLIA in frozen section

A

Victoria Blue

43
Q

enumerate basic fuchsin stains

A

Schiff’s
Carbol fuchsin
Coleman’s Feulgen
Aldehyde fuchsin/Gomori’s
Mallory’s fuchsin

44
Q

Enumerate cytoplasmic counterstains

A

RED
Eosin Y
Eosin B
Phloxine B
Rose Bengal

YELLOW
Picric acid
Orange G

GREEN
Light green SF
Lissamine green

45
Q

Enumerate nuclear counterstain

A

RED
Neutral red
Safranin
Carmine

BLUE
Methylene blue
Toluidine blue
Celestine blue
Hematoxylin

46
Q

What is Trypan Blue

A

Used in vital staining of reticuloendothelial system

47
Q

This staining demonstrates cytoplasmic phagocytosis

A

Vital staining

48
Q

Used to stain living cells immediately after removal from the living body

A

Supravital staining

49
Q

Done by injecting the dye into any part of the animal body (IV, subcutaneous, intraperitoneal) producing specific coloration of certain cells, particularly those of the RES

A

Intravital staining

50
Q

Used in supravital staining and is probably the best vital dye

A

Neutral red

51
Q

Stains used in intravital staining

A

Lithium
India ink
Carmine

52
Q

Metallic impregnation have the greatest application in tissue from the ___ and for the demonstration of ___

A

CNS, reticulin

53
Q

Stains used in metallic impregnation

A

Silver nitrate
Osmium tetroxide

54
Q

This is the most commonly used stain in metallic impregnation

A

Silver nitrate

55
Q

This staining entails the use of the specific dyes that stains tissues with a color that is different from that of the stain color itself

A

Metachromatic staining (metachromasia)

56
Q

Stains used in metachromasia

A

Metachromatic dye
Cresyl blue

57
Q

This stain is a basic dye belonging to Thizine and Triphenylmethane groups which stains for mast cell granules

A

Metachromatic dye

58
Q

Stain used for reticulocytes which stains it violetish-blue or deep blue

A

Cresyl blue

59
Q

Stains under histochemical staining

A

Perl Prussian Blue
Periodic Acid Schiff

Histopathology (16 decks)

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