Fixative uses Flashcards
satisfactory for routine paraffin sections
aldehyde fixatives
for central nervous tissue and postmortem tissues
10% formol saline
recommended for preservation and storage of surgical, post-mortem and research specimens
10% neutral buffered formalin or phosphate buffered formalin
recommended for routine post-mortem tissues
formal-corrosive
good for preservation of glycogen and micro-incineration techniqueused to fix sputum since it coagulates mucus
alcoholic formalin (genre’s fixative)
glutaraldehyde solution used for small fragments and needle biopsies fixed in 2-4 hrs at room temp
concentration
2.5% solution
glutaraldehyde solution used for larger tissues fixed in 6-8 hrs up to 24 hrs
4% solution
routine fixative of choice for preservation of cell detail in tissue photography
mercuric chloridemost common metalic fixative
recommended for fixing small pieces of liver, spleen, connective tissue fibers and nuclei
zenker’s fluid
excellent microanatomic fixative for pituitary gland, bone marrow, and blood containing organs such as spleen and liver
zenker-formol (helly’s solution)
main fixative for tumor biopsies especially the skin. excellent cytologic fixative
heidenhain’s susa
used for bone marrow biopsies
Lillie’s b5 fixative
precipitates all proteins and adequately preserves carbohydrates
chromic acid
preserves lipids and preserves mitochondria
potassium dichromate
recommended for demonstration of chromatin, golgi bodies, BC, and colloid containing tissues
regaud’s (muller) fluid
recommended for early degenerative process and tissue necrosis
orth’s fluid
demonstrates rickketsia and other bacteria
orth’s fluid
recommended for fixation of embryos and pituitary biopsies
bouin’s solution
normally used in conjunction with other fixatives to form a compound solution
glacial acetic acid
denatures and precipitates proteins by destroying hydrogen and other bonds. may be used both as fixative and dehydrating agent
alcohol fixatives
excellent for fixing dry and wet smears, blood smears and bone marrow biopsies
methyl alcohol 100%
used for fixing touch preparations
isopropyl alcohol 95%
recommended for fixing chromosomes, lymph glands, and urgent biopsies
carnoy’s fluid
recommended for fixing mucopolysaccharides and nuclear proteins
newcomer’s fluid
fixation with this solution causes complete denaturation of protein
osmium tetroxide
most common chrome-osmium acetic acid fixative uses
flemming’s solution
excellent fixative for nuclear structures and permanently fixes fat
flemming’s solution
recommended for cytoplasmic strucutres
flemming’s solution without acetic acid
recommended for study of water diffusible enzymes especially phosphatases and lipasesused in fixing brain tissues for diagnosis of rabies
acetone
usually for frozen tissue sections and preparation of bacteriologic smears
heat fixation