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Histopathology > Common stains > Flashcards

Common stains Flashcards

1
Q

Color of muscle fiber in HE stain

A

Deep pink

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2
Q

Color of cytoplasm and proteins (in edema fluid) in HE stain

A

Pale pink

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3
Q

Color of decalcified bone/osteoid/collagen in HE stain

A

Pink

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4
Q

Color of karyosome in HE stain

A

Dark blue

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5
Q

Color of cartilage in HE stain

A

Light blue to dark blue

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6
Q

Color of RBC/eosinophil granules/keratin in HE stain

A

Bright orange to red

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7
Q

Color of calcium and calcified bone in HE stain

A

Purplish blue

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8
Q

Color of plasma cells/osteoblast/basophilic cytoplasm in HE stain

A

Purplish pink

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9
Q

Color of nucleus in HE stain

A

Blue to blue black

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10
Q

This is a specialized fixative used in frozen section which serves to localize antigens and hydrolytic enzymes

A

Formol calcium

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11
Q

This is used for preservation of lipids

A

Formol calcium

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12
Q

It is similar with HE but differentiation stage is omitted. It is a progressive form of staining

A

Modified HE technique

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13
Q

This is composed of 3 stains

A

Papanicolaou stain

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14
Q

Stains used in papanicolaou stain

A

Hematoxylin- nuclear structures
OG-6 (orange green)- cytoplasm of mature cells
Eosin azure- cytoplasm of immature cells

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15
Q

Composition of eosin azure

A

Eosin
Bismarck brown
Lithium carbonate
PTA
Light green stain

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16
Q

This stain omitted the bismarck brown in eosin azure solution

A

Modified Pap’s stain

17
Q

Advantage of modified Pap’s stain

A

Sharpness of color and brilliant staining reactions are improved

18
Q

Where is haemotoxylin from

A

Core of heartwood tree (Haematoxylon campechianum)

19
Q

What is hematin

A

Oxidation of hematoxylin

20
Q

Most valuable staining reagent due to powerful nuclear and active coloring agent

A

Haematoxylin

21
Q

What should be added to hematoxylin for it to have high affinity for tissues

A

Mordant

22
Q

A red acid dye used for cytoplasm differentially

A

Eosin

23
Q

Commonly used as a background or contrast stain because it gives a leasing and useful contrast to nuclear stains (hematoxylin)

A

Eosin

24
Q

Most commonly used eosin

A

Eosin Y

25
Q

Rarely used Eosins

A

Eosin B and Eosin S/Ethyl Eosin

26
Q

Examples of mordants

A

Aluminum (most common; blue lake)
Chromium
Iron
Copper
Tungsten/PTA
Ferric salt (intense blue-black)

27
Q

This is a process of selective removal of excess dye

A

Differentiation

Histopathology (16 decks)

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