Common stains

Question 1

Color of muscle fiber in HE stain

Answer 1

Deep pink

Question 2

Color of cytoplasm and proteins (in edema fluid) in HE stain

Answer 2

Pale pink

Question 3

Color of decalcified bone/osteoid/collagen in HE stain

Answer 3

Pink

Question 4

Color of karyosome in HE stain

Answer 4

Dark blue

Question 5

Color of cartilage in HE stain

Answer 5

Light blue to dark blue

Question 6

Color of RBC/eosinophil granules/keratin in HE stain

Answer 6

Bright orange to red

Question 7

Color of calcium and calcified bone in HE stain

Answer 7

Purplish blue

Question 8

Color of plasma cells/osteoblast/basophilic cytoplasm in HE stain

Answer 8

Purplish pink

Question 9

Color of nucleus in HE stain

Answer 9

Blue to blue black

Question 10

This is a specialized fixative used in frozen section which serves to localize antigens and hydrolytic enzymes

Answer 10

Formol calcium

Question 11

This is used for preservation of lipids

Answer 11

Formol calcium

Question 12

It is similar with HE but differentiation stage is omitted. It is a progressive form of staining

Answer 12

Modified HE technique

Question 13

This is composed of 3 stains

Answer 13

Papanicolaou stain

Question 14

Stains used in papanicolaou stain

Answer 14

Hematoxylin- nuclear structures
OG-6 (orange green)- cytoplasm of mature cells
Eosin azure- cytoplasm of immature cells

Question 15

Composition of eosin azure

Answer 15

Eosin
Bismarck brown
Lithium carbonate
PTA
Light green stain

Question 16

This stain omitted the bismarck brown in eosin azure solution

Answer 16

Modified Pap’s stain

Question 17

Advantage of modified Pap’s stain

Answer 17

Sharpness of color and brilliant staining reactions are improved

Question 18

Where is haemotoxylin from

Answer 18

Core of heartwood tree (Haematoxylon campechianum)

Question 19

What is hematin

Answer 19

Oxidation of hematoxylin

Question 20

Most valuable staining reagent due to powerful nuclear and active coloring agent

Answer 20

Haematoxylin

Question 21

What should be added to hematoxylin for it to have high affinity for tissues

Answer 21

Mordant

Question 22

A red acid dye used for cytoplasm differentially

Answer 22

Eosin

Question 23

Commonly used as a background or contrast stain because it gives a leasing and useful contrast to nuclear stains (hematoxylin)

Answer 23

Eosin

Question 24

Most commonly used eosin

Answer 24

Eosin Y

Question 25

Rarely used Eosins

Answer 25

Eosin B and Eosin S/Ethyl Eosin

Question 26

Examples of mordants

Answer 26

Aluminum (most common; blue lake)
Chromium
Iron
Copper
Tungsten/PTA
Ferric salt (intense blue-black)

Question 27

This is a process of selective removal of excess dye

Answer 27

Differentiation