Common stains Flashcards
Color of muscle fiber in HE stain
Deep pink
Color of cytoplasm and proteins (in edema fluid) in HE stain
Pale pink
Color of decalcified bone/osteoid/collagen in HE stain
Pink
Color of karyosome in HE stain
Dark blue
Color of cartilage in HE stain
Light blue to dark blue
Color of RBC/eosinophil granules/keratin in HE stain
Bright orange to red
Color of calcium and calcified bone in HE stain
Purplish blue
Color of plasma cells/osteoblast/basophilic cytoplasm in HE stain
Purplish pink
Color of nucleus in HE stain
Blue to blue black
This is a specialized fixative used in frozen section which serves to localize antigens and hydrolytic enzymes
Formol calcium
This is used for preservation of lipids
Formol calcium
It is similar with HE but differentiation stage is omitted. It is a progressive form of staining
Modified HE technique
This is composed of 3 stains
Papanicolaou stain
Stains used in papanicolaou stain
Hematoxylin- nuclear structures
OG-6 (orange green)- cytoplasm of mature cells
Eosin azure- cytoplasm of immature cells
Composition of eosin azure
Eosin
Bismarck brown
Lithium carbonate
PTA
Light green stain
This stain omitted the bismarck brown in eosin azure solution
Modified Pap’s stain
Advantage of modified Pap’s stain
Sharpness of color and brilliant staining reactions are improved
Where is haemotoxylin from
Core of heartwood tree (Haematoxylon campechianum)
What is hematin
Oxidation of hematoxylin
Most valuable staining reagent due to powerful nuclear and active coloring agent
Haematoxylin
What should be added to hematoxylin for it to have high affinity for tissues
Mordant
A red acid dye used for cytoplasm differentially
Eosin
Commonly used as a background or contrast stain because it gives a leasing and useful contrast to nuclear stains (hematoxylin)
Eosin
Most commonly used eosin
Eosin Y
Rarely used Eosins
Eosin B and Eosin S/Ethyl Eosin
Examples of mordants
Aluminum (most common; blue lake)
Chromium
Iron
Copper
Tungsten/PTA
Ferric salt (intense blue-black)
This is a process of selective removal of excess dye
Differentiation