Monoengavirales - Rhabdoviridae

Question 1

Rhabdos meaning

Answer 1

Rhabdos = rod, stick -> plant rhabdoviruses

Question 2

Morphology

Answer 2

-ssRNA genome, helical capsid (?), enveloped, big size

non-segmented

Question 3

Complete virons

Answer 3

bullet shaped (B)

Question 4

Incomplete virons

Answer 4

Truncated (T)

70% of virons are incomplete

Question 5

Resistance

Answer 5

Weak

Question 6

Replication

Answer 6

Baltimore V - viral transcriptase

Question 7

Way of infections

Answer 7

cell-bound infections (=virions do not flow freely in the blood)

Question 8

CPEs

Answer 8

weak - IF if necessary (can’t follow propagation (replication) even though they propagate fast)

Question 9

fish vesiculovirus

Answer 9

thermosensitive

Question 10

Is zoonoses possible?

Answer 10

yes

Question 11

Antigenic properties

Answer 11

good antigens, cross-reactions within genera

Question 12

Diagnosis

Answer 12

• IF, isolation + IF
• experimental infection + IF
• RT-PCR, serology: VN (combined with IF, bc. there’s no CPE’s), ELISA

Question 13

Genus (20)

Answer 13

• Vesiculovirus
• Lyssavirus (->neves, not blood)
• Ephemerovirus
• Novirhabdovirus

Question 14

Vesiculovirus:

Vesicular stomatitis virus

Answer 14

ungulates, human (America)
clinical signs: similar to FMD
-can be mistaken in Bo and Sus, but if found in Eq it’s defidently Vesiculovirus (because Eq is not succeptible to Foot & Mouth disease)

Question 15

Vesiculovirus:
Spring viraemia (NF!)

Answer 15

affects carp

-> hemorrae, ascites - only in cold water

Question 16

Lyssavirus genus

meaning of lyssa

Answer 16

lyssa = rabies

Question 17

Genotypes

Answer 17

16, but only need to know Rabies, the others are mosly found in bats

Question 18

type of infection

Answer 18

neuroinvasive - lethal encephalitis: behavior changes
death when virus reaches the brain through the nervous sustem (time before death depends on where on the body infection starts - foot>face)

Question 19

Prevention

Answer 19

sereal vaccination

Question 20

urban rabies

Answer 20

dogs -> humans

Question 21

Sylvatic rabies

Answer 21

fox, wolf, raccoon, bat

Question 22

Accidental hosts

Answer 22

cat, horse, sheep, cattle

Question 23

Ephemerovirus genus:

Bovine ephemeral fever

Answer 23

short fever - milk production decreases

-mosquito-transmitted (Africa, Asia and Australia)

Question 24

Novirhabdovirus genus

• Viral haemorrhagic septicaemia
• Infectious haemopoetic necrosis

Answer 24

• Viral haemorrhagic septicaemia: Trous (Europe)

- Infectious haemopoetic necrosis: Salmon (Europe