Monocytes

Question 1

Monocyte Growth Factors

Answer 1

SCF, CSF-M, CSF-GM, IL-3, IL-6

Question 2

STAGES OF MONOPOIESIS

Monoblast
1. helps in identification
2. alternative
- stain marker for primitive myelocytic cell
- monocytic precursor are positive with this
> 2 substrates
3. stainable with ___ & ____ which will result to ____

Promonocyte
1. mitotically active and can divide until ____

Monocyte
1. nucleus:
2. cytoplasm: ground glass (_____)

Answer 2

Monoblast
1. CD-marker thru Immunologic Studies
2. Cytochemical stains
- Myeloperoxidase
- Non Specific Esterase
> alpha-naphthyl acetate esterase
> alpha-naphthyl butyrate esterase
3. Sudan B & Peroxidase = faintly positive or negative

Promonocyte
1. 3 more times

Monocyte
1. stringy/ lace-like
2. azure glass

Question 3

Kinetics of Monoycte

1. life span _____ circulation
2. granules (5)

Answer 3

1. 12-70hrs
2. Acid Phosphatase
   b-glucoronidase,
   lysozyme
   lipase
   peroxidase

Question 4

Specialized Macrophages
1. Kidney
2. Lungs
3. Brain
4. Skin
5. Tissue
6. Liver
7. lymph nodes
8. Intestinal wall

Answer 4

1. Mesangial cell
2. Alveolar macrophage
3. Microglial cells
4. Langerhans cells
5. Histiocyte
6. Kupffer cells
7. Follicular DCs
8. M-cells

Question 5

Functions of Monocytes/ Macrophage

1. make up the RES (?)/ MPS (?)
2. Macrophages in spleen do (2)
3. have receptor for arc portion of __ & ___
4. help regulate _____ (direct action of cell on destruction of cells) & _____ (fxn of plasma cells on producing antibodies)
5. monocyte has ___________ which they
   use to present antigen to the lymphocyte
   - lymphocyte will produce ______ (____) and activate other cells
6. major storage form of iron (2)
7. macrophage is surrounded by developing RBC trying to get iron in the storage cell

Answer 5

1. Reticulo-Endothelial System/ Mononuclear Phagocyte System
2. Spleening Culling & Spitting
3. IgG and c3
4. cellular activity; humoral activity
5. surface antigen presenting area
   - lymphokines (IL2)
6. Ferritin & Hemosiderin
7. Suckling/ Nursing Phenomenon

Question 6

Capability to Secrete
1. can kill virally infective cells (important protein against virus
2. important in transport of vitamin B12
3. precursor of plasmin that functions in clot lysis

Answer 6

1. Interferon
2. Transcobalamin II
3. Plasminogen

Question 7

Monocyte vs. Lymphocyte
1. Largest
2. Smallest
3. grayish blue; abundant
4. light sky blue; scarce
5. laced like chromatin
6. black chromatin
7. most important criteria in differentiating monocyte

Answer 7

1. Monocyte
2. Lymphocyte
3. Monocyte
4. Lymphocyte
5. Monocyte
6. Lymphocyte
7. chromatin