2.2

Question 1

FACTORS AFFECTING RBC PRODUCTION
1. inhibit EPO production
2. enhances EPO production
3. 2 vitamins that are important in synthesis of DNA
- disease affected by delayed nuclear maturation
4. important in Hgb synthesis (2)
5. protein needed in hgb production

Answer 1

1. Estrogen
2. Testosterone
3. Folic acid, Vitamin B12
   - megaloblastic anemia
4. Iron, Copper
5. Globin in Hgb

Question 2

1. ______ produces neutrophils
   - ___ > ____ > neutrophils
2. ______ produces eosinophils
3. ______ produces basophils
4. how many days from blast stage to release

Answer 2

1. CFU-G
   - CFU-GM > CFU-G > Neutrophil
2. CFU-Eo
3. CFU-Baso
4. 14 days

Question 3

STAGES OF GRANULOPOIESIS

1. more immature = larger nucleus; no granules
2. primary granules stars to appear
   - primary granules aka

Answer 3

1. Myeloblast
2. Promyelocyte
   - Azurophilic gramules

Question 4

STAGES OF GRANULOPOIESIS

1. number of Azurophilic granules starts to decrease
   - ____/_____ starts to appear
   - late in this stage, ______ starts to appear
   - secondary granules appear reddish tinge resulting now as if sun is coming out _______
   - unstained portion in this ^
2. nucleus starts to undergo identation
   - aka
   - further production of ____
   - how do you call these granules
   - basophil color
   - neutrophil color
   - eosinophil color

Answer 4

1. Myelocyte
   - Secondary granules or Specific granules
   - Tertiary granules
   - Dawn of Neutrophilia
   - golgi complex
2. Metamyelocyte
   - Juvenile cell
   - tertiary granules
   - gelatinase granules
   - dark blue to black
   - pink to lilac
   - orange red

Question 5

STAGES OF GRANULOPOIESIS

1. youngest stage to appear in circulation
   - nucleus undergoes ______
   - often __-shaped (3)
   - stage that normally appear in the circulation with the amount of at least ___ to __%
   - more than this %, indicates ______
2. Mature Neutrophil aka ______

Answer 5

1. Band/ Stab Cell/ Staff Cell
   - elongation
   - C,U,S- shaped
   - 0-5%
   - acute leukemia
2. Polymorphonuclear Neutrophil (PMN)

Question 6

How to differentiate:

1. myeloblast vs promyelocyte
2. promyelocyte vs myelocyte
3. myelocyte vs metamyelocyte
4. metamyelocyte vs band cell

Answer 6

1. cytoplasmic granulation
2. appearance of granules
3. shape of nucleus
4. nuclear morphology

Question 7

1. stages that are mitotically active
2. first stage incapable of mitosis

Answer 7

1. myeloblast > promyelocyte > myelocyte
2. metamyelocyte

Question 8

STAINS USED TO IDENTIFY CELLS
1. cytochemical stains (2)
2. these stains can differentiate _____ & ______
3. specific enzyme in peroxidase

Answer 8

1. Peroxidase
2. lymphocytic & erythrocytic
3. esterase

Question 9

N/C ratio
1. Myeloblast
2. Promyelocye

Nucleoli
1. Myeloblast
2. Promyelocye

Diameter
1. Myeloblast
2. Promyelocyte
3. Myelocyte
4. Metamyelocyte
5. Band Cell

Answer 9

N/C ratio
1. 4:1
2. 3:1 - 2:1

Nucleoli
1. 2-5
2. 2-3

Diameter
1. 15-20um
2. 15-21um
3. 12-18um
4. 10-15um
5. 9-15um