Module 4

Question 1

TRUE or FALSE:
Morphology of most viruses can be helpful in identifying the virus and assign unknown virus to the
correct family.

Answer 1

TRUE

Question 2

Late 1960s, __________________ was the means to the discovery of several new families of
previously noncultivable viruses

Answer 2

Electron Microscopy

Question 3

The modification is the “_________________” of the antibody with a fluorochrome that absorbs ultraviolet
light of a defined wavelength, but emits light at a higher wavelength.

Answer 3

Tagging

Question 4

Detection of viral antigens:
In which the emitted light is detected optically with a special microscope equipped with filters specific
for the emission wavelength of the fluorochrome.

Answer 4

Immunofluorescence Staining

Question 5

Detection of viral antigens:
In which the antigen is detected through the binding to the sample matrix of specially modified, agent
specific antibodies.

Answer 5

Immunofluorescence Staining

Question 6

Detection of viral antigens:
In principle, very similar is IF staining.

Answer 6

Immunohistochemical (immunoperoxidase) Staining

Question 7

Detection of viral antigens:
In which, sample is usually formalin-fixed

Answer 7

Immunohistochemical (immunoperoxidase) Staining

Question 8

Detection of viral antigens:
It can be kept for extended periods of time for several observations

Answer 8

Immunohistochemical (immunoperoxidase) Staining

Question 9

Immunohistochemical (immunoperoxidase) Staining requires more than how many hours to obtain results?

Answer 9

24 hours

Question 10

Detection of viral antigens:
Have moderate sensitivity.

Answer 10

Enzyme Immunoassay (EIA) – Enzyme-linked immunosorbent assay (ELISA)

Question 11

Detection of viral antigens:
Which is highly valuable especially in group settings (for herd status).

Answer 11

Enzyme Immunoassay (EIA) – Enzyme-linked immunosorbent assay (ELISA)

Question 12

Detection of viral antigens:
Mostly are solid-phase enzyme immunoassays.

Answer 12

Enzyme Immunoassay (EIA) – Enzyme-linked immunosorbent assay (ELISA)

Question 13

Detection of viral antigens:
That “Capture” antibody attached to a solid substrate typically the wells of polystyrene or polyvinyl microtiter plates.

Answer 13

Enzyme Immunoassay (EIA) – Enzyme-linked immunosorbent assay (ELISA)

Question 14

Detection of viral antigens:
Refers to the migration of antigen or antigen-antibody complexes through a filter matrix or in a lateral flow format.

Answer 14

Immunochromatography

Question 15

Detection of viral antigens:
In which, Antigen antibody complexes are then immobilized in the support matrix by an unlabeled
antibody bound to the matrix.

Answer 15

Immunochromatography