missed concepts Flashcards
what is hexosaminidase?
breaks down GM2; impairment of this enzyme is associated with tay sachs disease
what is a positive allosteric modifier
a molecule binds to an allosteric site and increases substrate affinity for the enzyme; associated with a left shift in a MM curve
Describe collagen I synthesis
- hydroxylation of proline residues in pro collagen
- assembly of pro collagen triple helix
- extracellular secretion of pro collagen
- cleavage of pro collagen peptides
describe the lamina lucida
the layer of the basement membrane closest to the epithelium. it contains glycoproteins like lamin and integrin
describe the lamina densa
a layer in the basement membrane closer to the underlying connective tissue. it is rich in type IV collagen and proteoglycans.
describe miRNA, lncRNA, and snRNA
miRNA: made by RNA polymerase II. acts as a “manager” in determining if proteins are translated
lnc RNA: can bind directly to DNA via complementary base pairing and impact chromatin remodeling and transcription
snRNA: post-transcriptional modification such as splicing
if given a coding strand, how do you find the transcribed RNA?
RNA strand will be identical with U replacing T
if given a template strand, how do you find the transcribed RNA?
find the complementary strand!
what is the function of HAT? HDAC?
HAT adds acetyl groups to histones, HDAC removes acetyl groups from histones
what base directly follows the kozak sequence?
G
describe catabolite repression
when glucose is high, CAP can’t be activated by cAMP! this prevents the lac operon from functioning
zinc fingers are associated with ___ receptors
hormone
the basic AA in bzip binds to…
cis-acting sequences
what features do prokaryotic mRNA not have
5’ cap, poly A tail, introns
describe an ABC transporter
transmembrane transporters with an ATP binding domain. they look like a V that opens and closes.
