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Cell Biology > Microscopy + Visualisation > Flashcards

Microscopy + Visualisation Flashcards

1
Q

Light bends when it passes through a lens. This is called…

A

Refraction

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2
Q

Using the light microscope you can see…

A

Cells!

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3
Q

The light microscope gives a …

A

Enlarged inverted image

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4
Q

The lens in the eyepiece of the light microscope is called the…

A

Ocular lens!

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5
Q

What controls how much light passes through the stage?

A

Diaphragm

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6
Q

Which objective lens should you use when you FIRST start focusing your specimen?

A

4x

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7
Q

Resolution is determined by…

A

Wavelength of radiation and NA of objective

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8
Q

Limit of resolution of the light microscope is…

A

200nm

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9
Q

The NA or Numerical Aperture describes the… of an objective.

A

Ability to collect light

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10
Q

Which objective must NEVER be used with the coarse adjustment knob?

A

100x

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11
Q

What is the function of the condenser lens?

A

Focuses light through the specimen!

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12
Q

A microscope has 10x ocular lens and 40x objective, what is the total magnification?

A

400x

10 x 40 = 400!

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13
Q

Which type of microscope would allow you to see the 3D SURFACE of bacteria and viruses?

A

Scanning electron microscope

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14
Q

Which type of microscope gives the greatest magnification?

A

Electron microscope

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15
Q

The MAIN reason that the electron microscope gives a BETTER resolution than the light is…

A

The shorter wavelength of an electron beam

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16
Q

… waves have the highest / longest wavelength

17
Q

Which type of microscope uses UV light?

A

Fluorescence microscope

18
Q

On the objective lens what is number written next to the word oil?

A

Numerical Aperture

19
Q

What is the 2nd number written on the objective?

A

Coverslip thickness

20
Q

What is the FIRST step of tissue preparation?

21
Q

What is the most common fixative used?

How does it preserve a specimen?

A

Formalin

By cross linking proteins!

22
Q

After embedding the specimen, what is the first thing you need to do before you stain it?

A

Cut it into thin sections

23
Q

The organic dye Eosin is used to stain…

A

The cytoplasm

24
Q

In Immunohistochemistry, the DIRECT method used a primary and secondary antibody.
True or false?

25
Q

This stain works well with staining carbohydrates…

A

PAS (periodic acid Schniff reaction)

26
Q

In the PAS staining of the Duodenum what are the purple structures labelled A?

A

Goblet cells!

Cell Biology (10 decks)

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