Microscopy - AnaPhy Lab Flashcards
is an essential tool in the study of human anatomy and physiology
compound light microscope
The human eye is not able to perceive objects smaller than
0.1 mm in diameter
it requires a light source in order to view an object.
light microscope
meaning that once you focus the specimen using one objective, it will remain “almost” in focus when viewed through another objective
parfocal
The major parts of a microscope—the parts that form its framework—
the base, the arm, and the head
The upper part of the microscope that supports the ocular lens(es) and the various objective lenses
Head
The broad, flat, lower part of the microscope that supports the rest of the instrument.
Base
The vertical part of the microscope that connects the head to the base.
Arm
Lens(es) located within the eyepieces
Ocular lenses
have one ocular lens
Monocular microscopes
have two ocular lenses
binocular microscopes
Ocular lenses typically magnify an object
10 times
Magnifying lenses mounted on a rotating nosepiece.
Objective lenses
Most microscopes have four objective lenses:
scanning (4×), low-power (10×), highpower (40×), and oil-immersion (100×) lenses.
Connects the objective lenses to the head and allows different objective lenses to be moved into place.
rotating nosepiece
Flat, horizontal shelf onto which the slide is placed and typically secured with a spring clamp. Two control knobs can be used to move the stage to position the slide.
Mechanical stage
Small lens located under the stage that concentrates light onto the specimen
Condenser
is used to raise and lower the condenser
condenser adjustment knob
The condenser should usually be in its ______________ position, just __________ the aperture
uppermost; below
the hole in the stage through which light travels
aperture
Located beneath the condenser. Regulates the
amount of light that passes through the condenser.
Iris diaphragm lever
Located on the arm of the microscope just above the base
focus knobs
moves the stage up and down in large increments and is
used to find the specimen and for initial focusing
coarse adjustment knob
is used for fine focusing after coarse focusing has been completed
fine adjustment knob
Located in the base. Provides the light that passes through the condenser, the specimen, the lenses, and finally into your eyes
Substage light
located on the base or the arm controls the brightness of the light
light control knob