Microscopy Flashcards
Microscopy is done for what purposes
What are the types of microscopes?
Optical /light microscope
Phase contrast microscope
Dark field microscope
Fluorescent microscope
Electron microscope
The light microscope contains what?
A light source and a compound lens system
The lens system consists of what?
Of a number of objective lenses in conjunction with a fixed, ocular lens (eye piece)
Light microscope are equipped with what power objective lenses?
Visualization of bacteria generally requires what?
The use of immersion oil with X100 objective
X 40 objective is used for what ?
Describe the phase contrast microscope
describe the dark field
The contrast of the dark field gives what?
An illusion of increased resolution
The dark field microscope is used for what organisms
Spirochetes
Fluorescence is phenomenon that occurs when?
In fluorescent microscope specimens are exposed to what?
To a light shorter wavelength (ultra violet), which results in emission of longer wavelength visible light
How are bacteria visible when stained with fluorescent dye
Visible as brightly glaring objects in dark background
Fluorescent microscopy is used for what?
For detection of antigen and antibodies
Describe the electron microscope
