Growth, Nutrition And Metabolism Of Bacteria Flashcards

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1
Q

The minimum nutritional requirement for growth and multiplication of bacteria include sources of what

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2
Q

What is the classification of bacteria on basis of nutrition

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3
Q

How does the bacteria divide

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4
Q

What happens after the two identical DNA molecules are formed ?

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5
Q

What is generation time

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6
Q

Why is bacterial multiplication is arrested after a few cell divisions

A

Due to exhaustion of nutrients and accumulation of toxic products

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7
Q

What is total count

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8
Q

What is viable count

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9
Q

What are the phases of the growth curve

A

Lag phase
Log phase
Stationary phase
Phase of decline

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10
Q

Explain lag phase

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11
Q

Explain log phase

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12
Q

Explain stationary phase

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13
Q

Explain phase of decline

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14
Q

What are the products of bacterial growth

A

Toxins
Extracellular enzymes
Pigment

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15
Q

List the toxins produced by bacterial growth

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16
Q

What are the Extracellular enzymes produced as the result of the bacterial growth

A

Coagulase - clot plasma, if present bacteria pathogenic
Hyaluronidase
Streptokinase - converts plasminogen to plasmin
Hemolysis, leucocidin
IgA1 protease - breakdown of immunoglobulin antibodies

17
Q

What are the pigments produced as the result of bacterial growth

A

Localized and generalized pigmentation

18
Q

What is the principle constituent of the bacterial cell?

A

Water, which is about 80% of the total weight

19
Q

What is the minimum nutrition requirement for the growth of bacteria

A

Water, a source of carbon, nitrogen, and some organic salts

20
Q

These nutritional elements may be supplied in what

A

Suitable artificial culture media

21
Q

What are additional nutritional requirements for bacteria

A

Sulphur, phosphorus, and other elements such as sodium, potassium, magnesium in small amounts

22
Q

What do fastidious bacteria require

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23
Q

What are the environmental factors affecting growth

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Moisture and desication
Oxygen
Carbon dioxide
Temperature
pH
Light
Osmotic effect

24
Q

How does moisture affect the growth

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25
Q

How does oxygen affect the growth

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26
Q

How does carbon dioxide affect the growth

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27
Q

Which organism requires much higher levels of carbon dioxide

A

Brucella (5-10%) of carbon dioxide

28
Q

How does temperature affect the growth

A

The optimum range varies with different bacterial species. Most of the pathogenic bacteria grow at 37 degrees centigrade

29
Q

What are bacteria grouped into on the basis of optimum temperature range for the growth

A
30
Q

How does the pH affect the growth

A

Most of the medically important bacteria grow at pH 7.2-7.6

31
Q

How does the light affect the growth

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32
Q

Which bacteria produces pigment only on exposure to light

A

Photochromogenic mycobacteria

33
Q

How does osmotic effect affect the growth

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34
Q

What is metabolism

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35
Q

What does metabolism include

A

Oxidation
Fermentation

36
Q

Explain oxidation in bacterial metabolism

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37
Q

Explain fermentation in bacterial metabolism

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38
Q

What are the organic end products of fermentation

A

Acids, gas and alcohols