Microscopes

Question 1

Compared to other microscopes light miscroscopes are worse because

Answer 1

Lower resolution and magnification

Question 2

However the benefit of light microscopes are

Answer 2

Can view live objects

Question 3

Slide preparation of light microscopes

Answer 3

Simple - sliced samples and staining

Question 4

Advantages of light microscopes

Answer 4

Cheap, easy to use, view live specimens

Question 5

Disadvantages of light microscope

Answer 5

Low resolution - can’t see ribosomes
Poor surface view

Question 6

Transmission electron microscope resolution and magnification

Answer 6

x1,000,000
Less than 1nm

Question 7

Slide preparation for transmission electron

Answer 7

Complex - fixed with chemicals or freezing, stained with heavy metals and dehydrated and in resin

Question 8

Advantages of transmission electron

Answer 8

Powerful magnification
Very detailed

Question 9

Disadvantages of transmission electrons

Answer 9

Limited scanning

Question 10

Scanning electron resolution and magnification

Answer 10

X3,000,000
1 to 20 nm

Question 11

Slide preparation of scanning electron

Answer 11

Complex - fixed, stained, dehydrated and fractured

Question 12

Advantages of scanning electrons

Answer 12

High resolution
Valuable to industries

Question 13

Disadvantages of scanning electrons

Answer 13

Expensive
Large
Needs steady voltage

Question 14

How are electron miscroscopes compared to light

Answer 14

Light can view live organisms but electron only view dead ones