DNA replication Flashcards
1
Q
What enzyme catalyses the breaking of hydrogen bonds
A
Helicase
2
Q
Which enzyme catalyses the joining of nucleotide by phosphodiester bonds
A
Polymerase
3
Q
What is a semi conservative replication
A
Each newly synthesised DNA molecule is made up of 1 original DNA strand and one new one
4
Q
What reactions takes place to join nucleotides during semi conservative replication
A
Condensation
5
Q
Steps of DNA replication
A
- Helicase breaks hydrogen bonds to unwind the double helix
- Free nucleotides move to form hydrogen bonds with their complementary bases
- polymerase join free nucleotides by phosphodiester bonds to from the backbone
- Two identical copies made
6
Q
History of the finding of semi conservative replication
A
3 types of investigations from E Coli bacteria with heavy and light nitrogen
7
Q
History of the finding of semi conservative replication
A
3 types of investigations from E Coli bacteria with heavy and light nitrogen
