microscopes Flashcards
what is the magnification
the number of times greater the image you see is compared to the actual specimen
what is the resolution
the ability to distinguish between 2 points on an image. The higher the resolution, the clearer the image
explain what a light microscope is
Uses light reflections to magnify an object and has a lower resolution
explain what a electron microscope is
Uses a beam of electrons to magnify an object, has a higher resolution and there is 2 types
what are the 3 steps to prepare a slide
1) Place a small slice/ smear the specimen onto a glass
2) Place a small drop of water/ stain onto the specimen
3) Slowly lower a cover slip onto the specimen, trying to avoid air bubbles forming
what are the 4 different main types of stain
iodine, gram stains, eosin and methylene blue
what is the equation to calculate the magnification
magnification = image size/ actual size