Microscope Flashcards
Basic Unit of Metric System?
Meters
The Ocular is always…?
10
How to get the total magnification?
-Ocular (aka the eyepiece) x objective lens
How to get FOV diameter of a microscope? (Low power)
- Put clear ruler under the microscope
- Each line= 1mm
- Use under low power!
FOV diameter of higher magnifications?
(Low power FOV) x (Low power MAG)
______________________________
High Power MAG
Estimating the Size of the Specimen Under Observation?
1)Determine the fraction of the FOV that you estimate the sample occupies
2) Multiply the fraction by the size of the FOV
(For LP: FOV is determined by just the measurement)
(For HP: Fov is determined by the calculation)
Resolution
Ability to form separate images of objects that are very close together
Resolving Power
minimum distance 2 points can be separated and still be distinguished as separate points
The smaller the resolving power…
the better the resolution
3 Maximum resolutions of a light microscope vs electron microscope
light microscope: resolve to about 200 nm apart.
electron microscope: can resolve to about 0.2 apart (1000x better than light)
3 Example structures that are visible with electron microscopes but not with a light microscope
-Electron microscopes have stronger magnification and better resolution and can produce images with better detail
-EX: Golgi apparatus, cytoskeleton, endoplasmic
reticulum
How does an improvement in apparatus allow for greater understanding of cell structure?
The invention of the electron microscope led to better resolution when viewing cells
EX: mitochondria was discovered
How to focus the microscope on a sample
- Center the sample
- Increase MAG by moving to a HP objective lens
- Adjust amount of light
Define Micrograph
A photo taken through a microscope to show a magnified image of the item
State why the magnification of a drawing/micrograph is not the same as the magnification of the microscope
We draw structures much larger than we view them under a microscope.
