Microbiology Stuff

Question 1

Bugs that won’t Gram stain include:

Answer 1

• Treponema, Leptospira - too small
• Mycobacteria - cell wall of lipids
• Mycoplasma, Ureaplasma - no cell wall
• Legionella, Rickettsia, Chlamydia, Bartonella, Anaplasma, Ehrlichia - intracellular organisms

These Little Microbes May Unfortunately Lack Real Color But Are Everywhere.

Question 2

Bugs that Giemsa stain:

Answer 2

chlamydia, borrelia, rickettsia, trypanosomes, plasmodium

Certain Bugs Really Try my Patience.

Question 3

PAS+ organism:

Answer 3

Tropheryma whipplei

Question 4

Acid-fast/auramine-rhodamine organisms:

Answer 4

mycobacteria, Nocardia, protazoa

Question 5

India ink/mucicarmine used for:

Answer 5

Cryptococcus neoformans

Question 6

Bugs that silver stain:

Answer 6

Legionella, H. pylori, Fungi

Question 7

chocolate agar + V (NAD+) and X (hematin)

Answer 7

H. influenzae

Question 8

Thayer-Martin + vanc, TMP, colistin, nystatin

Answer 8

N. gonorrhoeae, meningitidis

Question 9

Bordet-Gengou agar, Regan-Lowe medium

Answer 9

B. pertussis

Question 10

Tellurite agar, Loffler medium

Answer 10

C. diptheriae

Question 11

Lowenstein-Jensen agar

Answer 11

M. Tb

Question 12

Eaton agar + cholesterol

Answer 12

M. pneumoniae

Question 13

MacConkey agar

Answer 13

lac+ enterics

Question 14

eosin-mythylene blue agar

Answer 14

E. coli (turn green)

Question 15

charcoal yeast extract agar + cysteine, Fe

Answer 15

Legionella

Question 16

Sabouraud agar

Answer 16

fungi

Question 17

aeorobes

Answer 17

Nocardia, Pseudomonas, M. tb

Nagging Pests Must Breath.

Question 18

obligate anaerobes

Answer 18

Clostridium, Bacteroides, Fusobacterium, Actinomyces

Can’t Breath Fresh Air.

Question 19

facultative anaerobes

Answer 19

Strep, Staph, Gram positive enterics

Question 20

obligate intracellular

Answer 20

Rickettsia, Chlamydia, Coxiella

you stay inside when it’s Really Chilly and Cold.

Question 21

facultative intracellular

Answer 21

Salmonella, Neisseria, Brucella, Mycobacterium, Listeria, Francisella, Legionella, Yersinia

Some Nasty Bugs May Live FacultativeLY.

Question 22

encapsulated bacteria

Answer 22

Pseudomonas, Strep pneumo, H. influenzae, N. meningitidis, E. coli, Salmonella, K. pneumoniae, GBS

Please SHiNE my SKiS.

Question 23

urease-positive organisms

Answer 23

Proteus, Cryptococcus, H. pyloria, Ureaplasma, Nocardia, Klebsiella, S. epidermidis, S. saprophyticus

Pee CHUNKSS (higher urinary pH from ammonia leads to struvite stones)

Question 24

catalase-positive organisms

Answer 24

Nocarida, Pseudomonas, Listeria, Aspergillus, Candida, E. coli, Staph, Serratia, B. cepacia, H. pylori

Cats Need PLACESS to hide and Belch Hairballs.