Microbiology Lesson 7

Question 1

Singular form of media

Answer 1

medium

Question 2

mixtures of nutrients that the microbes need to live. Also provides a surface and the necessary moisture and pH to support microbial growth.

Answer 2

Media

Question 3

is the medium that we most often use. Complex nutrient media which supports the growth of a wide variety of microbes.

Answer 3

Tryptic Soy Agar (TSA)

Question 4

SPECIALIZED MEDIAS

Answer 4

MacConkey’s Agar (MAC), Mannitol Salt Agar, & Blood Agar

Question 5

encourages some microorganisms to grow while stopping others. It does this by using specific ingredients.

Answer 5

Selective Media

Question 6

tells different microorganisms apart based on their appearance, like color or size. This allows scientists to identify specific types of microbes in a mix.

Answer 6

Differential Media

Question 7

Infection in lining of small intestine caused by bacteria (both G+ & G-), including Salmonella and Shigella.

Answer 7

Food poisoning

Question 8

Ingesting foods and materials that are fecally contaminated. Symptoms / Course: Diarrhea, fever, and abdominal cramps 12 - 72 hours after infection. Usually lasts 4 to 7 days. Most recover without treatment. Severe infections may last several weeks.

Answer 8

Transmission

Question 9

Replace fluids. Don’t use anti-diarrheals. May prolong illness.
Thorough cooking kills these bacteria. Proper food handling, storage and good hand washing are preventive measures.

Answer 9

Treatment

Question 10

most common staph infections. Approximately 20-30% of general population “Staph Carries.”

Answer 10

Staphylococcus aureus (golden staph)

Question 11

One of the four most common causes of nosocomial infections, often causing postsurgical wound

Answer 11

MRSA

Question 12

______ is normal flora which inhabit the skin of healthy humans.

Answer 12

S. epidermidis

Question 13

Most specimens received in a clinical microbiology lab are plated. It is an enriched medium that will grow even fastidious bacteria. Contains 5% sheep blood. Is usually inoculated from a patient’s throat swab

Answer 13

Blood Agar (BAP)

Question 14

Enzymes lyse the blood cells completely, producing a clear area around the colony

Answer 14

Beta-hemolysis (β-hemolysis)

Question 15

Incomplete hemolysis produces a greenish discoloration around the colony.

Answer 15

Alpha-hemolysis (α-hemolysis)

Question 16

• No effect on the red cells

Answer 16

Gamma-hemolysis (γ hemolysis)

Question 17

cause diseases including strep throat, necrotizing fasciitis (flesh eating bacteria), scarlet fever, postpartum fever, and streptococcal toxic shock syndrome.

Answer 17

Group A streptococci (S.pyogenes)

Question 18

can cause life threatening pneumonia and meningitis in newborns, the elderly and adults with compromised immune system.

Answer 18

Group B streptococci (S. agalacitiae)