Micro - Bacteria Flashcards
catalase test
GP
staph (+)
strep (-)
coagulase test
GP
staph aureus (+) staph epidermidis or saprophyticus (-)
novobicin test
GP
staph epidermidis (sens) staph saprophyticus (resis)
hemolysis
GP
streptococci (a, ß, g)
alpha hemolysis
GP
Strep. pneumoniae (capsule)
Strep. viridans (no capsule)
- differentiate btwn the two using optochin + bile solubility
ß hemolysis
GP
Strep pyogenes (grpA)
Strep agalactiae
Staph aureus
Listeria monocytogenes
LAAP = listeria, aureus, agalactiae, pyogenes
gamma hemolysis
GP
enterococcus
non-enterococcus
optochin sensitivity
GP
Strep. pneumoniae - sensitive
Strep. viridans - resistant
bile solubility
GP
Strep. pneumoniae - soluble (lysed by bile)
Strep. viridans - not lysed by bile
bacitracin
GP
S. pyogenes (sensitive)
S. agalactiae (resistant)
growth in bile and 6.5% NaCl
GP
Enterococcus faecalis
growth in bile, not 6.5% NaCl
GP
Strep. bovis
B cells typically infected by this virus
EBV
Ganglion cells typically infected by this virus
herpes
oligodendrocytes typically infected by this virus
JC virus –> PML
Measles –> SSPE
follicular dendritic cells typically infected by this virus
HIV
grows at 42˚C
GN, oxidase +, comma shaped
campylobacter jejuni
grows in alkaline media
GN, oxidase +, comma shaped
vibrio cholera
produces urease
GN, oxidase +, comma shaped
helicobacter pylori
GN diplococci
2
neisseria meningitidis
neisseria gonorrhoeae
differentiate using maltose test
maltose test is used for..
neisseria MeninGitidis (fermenter - maltose + glucose) neisseria Gonorrhoeae (non-fermenter - glucose only)
GN “coccoid” rods
4
haemophilus influenza (requires factor V, X)
Bordtella pertussis
Pasturella - animal bites
Brucella
Requires factor V, X
haemophilus influenza - GN coccoid rods
can also be grown with staph aureus, which provides factor V
lactose fermenters (rate) - Macconkey’s
GN
klebsiella, e. coli, enterobacter (fast fermenter)
citrobacter, serratia (slow fermenter)
MaconKEES