Mico bacteria lab algorithm Flashcards
actinomyces
gram positive
branching filament
anaerobic not acid fast
nocardia
gram positive
branching filament
aerobic acid fast
clostridium
gram positive
rod/bacillus
anaerobe - CANT breath o2
corynebacterium
gram positive
rod/bacillus
listeria
gram positive
rod/bacillus
mycobacterium
gram positive
rod/bacillus
acid fast
bacillus
gram positive
rod/bacillus
aerobe - BREATHES o2
staph epidermidis
coagulase negative novobiocin sensitive (NESSIR)
staph saprophyticus
coagulase negative novobiocin resistant (NESSIR)
staph aureus
coagulase positive
strep pneumo
alpha hemolytic - partial (green)
capsule (positive quellung)
optochin sensitive (OVERPASS)
viridans strep
AKA strep mutans
alpha hemolytic - partial (green)
no capsule
optochin resistant (OVERPASS)
strep pyogenes
beta hemolytic - complete (clear)
group A
Bacitran sensitive (B-BRAS)
strep agalactiae
beta hemolytic - complete (clear)
group B
bacitran resistant (B-BRAS)
enteroccocus
gamma hemolytic - no hemolysis
group D
Enterococcus faecalis
gamma hemolytic - no hemolysis
growth in bile + 6.5% NaCl
strep bovis
gamma hemolytic - no hemolysis
nonenteroccocus
growth in bile, not 6.5% NaCl
STAPHYLOCCOCI
gram positive coccus catalase positive (clusters)
STREPTOCOCCUS
gram positive coccus catalase negative (chains)
bacitran sensitive
B-BRAS
Group A strep (strep pyogenes)
bacitran resistant
B-BRAS
Group B strep (strep agalactiae)
optochin sensitive
OVERPASS
strep pneumo
optochin resistant
OVERPASS
viridans strep
novobiocin sensitive
NESSIR
staph epidermidis
novobiocin resistant
NESSIR
staph saprophyticus
alpha hemolytic bacteria
green ring
strep pneumo + viridans strep
beta hemolytic bacteria
clear ring
group A strep (strep pyogenes) + group B strep (strep agalactiae)
staph aureus
tumbling motility, meningitis in newborns, unpasteurized milk
listeria monocytogenes - beta hemolytic
campylobacter jejuni
gram negative, oxidase positive/comma shaped, grows in 42degC
vibrio cholerae
gram negative oxidase positive/comma shpaed, grows in alkaline media
psueodomonas + h pylori
gram negative rod lactose nonfermenter, oxidse positive
shigella, salmonella + proteus
gram negative rod lactose nonfermenter oxidase negative
citrobacter + serratia
gram negative rod SLOW lactose fermenter
klebsiella e coli + enterobacter
gram negative rod FAST lactose fermenter
h flu, pastuerella, brucella, bordetella pertusses
gram negative coccoid rods
neisseria meningitis
gram negative diplococci maltose fermenter
neisseria gonorrhea
gram negative diplococci maltose nonfermenter
lactose fermenting enteric bacteria
klebsiella, e coli enterobacter
slow - serratia + citrobacter
PINK on macConkeys agar
EMG agar - purple/black colonies
produces beta-galactosidase
e coli
lactose –> glucose + galactose
grows purple colonies w/ green sheen on EMB agar
e. coli
resistant to penicillin G and vancomycin
gram negative