METHODS OF INOCULATION

Question 1

deliberately introducing biological material to a culture medium for the purpose of stimulating growth or to a body for triggering immune response

Answer 1

Inoculation

Question 2

Biological material that is introduced to the medium or in the body

Answer 2

Inoculum

Question 3

• a.k.a Streak for Isolation/Quadrant Streaking
• progressively ‘thinning out’ of inoculum resulting in cells separating
• leads to discrete or isolated colonies

Answer 3

Clock Streaking

Question 4

• used for isolating microorganisms from the surface of materials
• The material is pressed over the surface of the medium for some time.
• The distribution of the mcgs can be traced after incubation

Answer 4

Imprint Method

Question 5

• used for sampling surrounding air/environment or aerosol
• plate is opened and exposed directly to the environment for a certain amount of time
• passive method of air sampling

Answer 5

Direct Plate Exposure

Question 6

• a small volume of liquid inoculum is spread over the surface of a solid medium using a sterile
  bent glass rod, also called a hockey stick (L-rod)
• results in a lawn plate with confluent growth

Answer 6

Spread Plate

Question 7

• flooding a solid media with a liquid inoculum and removing the excess using a pasteur pipette
• results in a lawn plate with confluent growth

Answer 7

Flood Plate

Question 8

• inoculum is delivered in an empty, sterile plate, after which sterile melted agar is poured until the bottom of the plate is completely covered
• results in subsurface and surface colonies

Answer 8

Pour Plate

Question 9

METHODS OF INOCULATION
media in tubes

Answer 9

Streaking an agar slant

Stabbing an agar butt

Question 10

Motility medium is prepared as butt agar to observe if the microorganism will spread throughout the tube

Answer 10

Stabbing an agar butt

Question 11

Scale up of inoculum during Bioprocessing

Answer 11

small scale
bench scale
pilot/production scale