ASEPTIC TECHNIQUE Flashcards

1
Q

developed the Germ Theory of Diseases
Mcgs leads to spoilage
Stipulated that the infections are caused by germs

A

Louis Pasteur (1860-1861)

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2
Q

used carbolic acid as antiseptic for surgeons hands and instruments
Reduced in surgical mortality (from 45% to 15%) in his accident ward

A

Joseph Lister (1865-1869)

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3
Q

germ barriers could also be achieved through heat were

A

sterilization, soap and water, and disinfectants

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4
Q

year germ barriers could also be achieved

A

1890s

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5
Q

destruction of germs in a surgeon’s hands, instruments or immediate surroundings using antiseptic washes.

A

Antisepsis system

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6
Q

procedures that prevents the contamination of specimens being worked on.

Also prevents the spread of mcgs from the specimen to the surroundings and to the handler.

A

Aseptic Technique

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7
Q

Treat your workbench/table top with what contents of disinfectant

A

(2.5% hypochlorite, 200 ppm quaternary ammonium compounds or 70% alcohol)

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8
Q

Sterile or vessels containing cultures are kept closed and opened only for a minimum amount of time needed for access.

A

true

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9
Q

enclosed, ventilated laboratory workspace for safely working with microorganisms.

A

biosafety cabinets

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10
Q

Mouths of opened vessels are briefly passed through a flame.

A

true

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11
Q

are commonly used for transferring inoculum. These are sterilized by flame.

A

Wire needles or loops

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12
Q

Common mistakes when using wire loops or needles

A

● Not flaming the wire long enough (not red hot)
● Not allowing the wire to cool
● Forgetting to flame the wire in between inoculations
● Placing the wire loop in the workbench while doing the experiment
● Spattering and aerosol formation due to residual inoculum

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13
Q

Larger volumes of liquid can be transferred using sterile

A

Pasteur pipettes or graduated pipettes

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14
Q

When using a sterile glass pipette. Hold the plugged ends only and avoid touching other portions of the pipette

A

true

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15
Q

glass pipettes or pipette tips are to be placed in a container with

A

disinfectant

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