ASEPTIC TECHNIQUE Flashcards
developed the Germ Theory of Diseases
Mcgs leads to spoilage
Stipulated that the infections are caused by germs
Louis Pasteur (1860-1861)
used carbolic acid as antiseptic for surgeons hands and instruments
Reduced in surgical mortality (from 45% to 15%) in his accident ward
Joseph Lister (1865-1869)
germ barriers could also be achieved through heat were
sterilization, soap and water, and disinfectants
year germ barriers could also be achieved
1890s
destruction of germs in a surgeon’s hands, instruments or immediate surroundings using antiseptic washes.
Antisepsis system
procedures that prevents the contamination of specimens being worked on.
Also prevents the spread of mcgs from the specimen to the surroundings and to the handler.
Aseptic Technique
Treat your workbench/table top with
disinfectant example
(2.5% hypochlorite, 200 ppm quaternary ammonium compounds or 70% alcohol)
Sterile or vessels containing cultures are kept closed and opened only for a minimum amount of time needed for access.
true
enclosed, ventilated laboratory workspace for safely working with microorganisms.
biosafety cabinets
Mouths of opened vessels are briefly passed through a flame.
true
are commonly used for transferring inoculum. These are sterilized by flame.
Wire needles or loops
Common mistakes when using wire loops or needles
● Not flaming the wire long enough (not red hot)
● Not allowing the wire to cool
● Forgetting to flame the wire in between inoculations
● Placing the wire loop in the workbench while doing the experiment
● Spattering and aerosol formation due to residual inoculum
Larger volumes of liquid can be transferred using sterile
Pasteur pipettes or graduated pipettes
When using a sterile glass pipette. Hold the plugged ends only and avoid touching other portions of the pipette
true
glass pipettes or pipette tips are to be placed in a container with
disinfectant
