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LBYBI13 > Culture media > Flashcards

Culture media Flashcards

1
Q

Mixtures composed of nutrients intended for the growth and identification of microorganisms

Energy source, C- and N-source, Salts, Optimum pH, Growth factors

Isolation, differentiation, identification, characterization, studying of responses/behavior

A

CULTURE MEDIA

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2
Q

CLASSIFICATION OF CULTURE MEDIA
1.5% to 2.0% agar

A

Solid

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3
Q

CLASSIFICATION OF CULTURE MEDIA
0.5% to 1.0% agar

A

Semi-solid

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4
Q

CLASSIFICATION OF CULTURE MEDIA
no agar

A

Liquid

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5
Q

came from the Malay word “agar-agar” which is the local word for the red algae in which the gel is harvested from

A

Agar

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6
Q

CLASSIFICATION OF CULTURE MEDIA
By application/use

A

Differential media
Selective media
Enrichment media
Enriched media
Simple media

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7
Q

what kind of media is used for the cultivation of non-fastidious microorganisms

A

Simple media

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8
Q

Simple media example

A

Nutrient Agar
Nutrient Broth
Potato dextrose agar

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9
Q

contains additional nutrients for the growth of fastidious microorganisms

A

Enriched media

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10
Q

are those that require special/particular nutritional requirements

A

Fastidious mcgs

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11
Q

Enriched media examples

A

Sheep blood, calf brain infusion, heart infusion broth

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12
Q

contain nutrients that allow a particular group of organisms that might not be present in sufficient numbers in the specimen to be isolated and identified

A

Enrichment media

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13
Q

Enrichment media examples

A

Selenite F - enrichment S. enterica serotype Typhi from fecal samples Tetrathionate broth - for Salmonella from feces and sewage

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14
Q

inhibit the growth of certain bacteria while permitting the growth of others

A

Selective media

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15
Q

Examples of Selective media

A

Mannitol Salt Agar (MSA)
Brilliant Green Lactose Broth (BGLBB)
Potato Dextrose Agar acidified w 10% tartaric acid

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16
Q

media that can distinguish one group or species from another. They have components that make observable changes when a certain biochemical occurs

A

Differential media

17
Q

Examples of Differential media

A

MacConkey Agar (MAC)
Eosin Methylene Blue Agar (EMBA)
Blood Agar Plate (BAP)

18
Q

Culture media is typically prepared as:

A

broth tube
agar slant
butt
butt slant
agar plate

19
Q

When preparing a batch of media consider the following:

A

Number of setups/treatments
Number of replicates
Number of controls
The media components
Type of set-ups e.g. plates, slants, etc.

20
Q

Adjust the pH using diluted _______.

A

HCl or NaOH

21
Q

For nutrient agar, it is recommended to adjust it to ______ (pH)

A

6.8

22
Q

Plates

A

Platesautoclave sterilize, then cool to 50 °C the pour to plates.

23
Q

Tubes sterilization

A

Distribute to tubes, autoclave sterilize, then form to a butt, butt-slant or slant.

24
Q

sterilization of Heat stable components

A
  • Steam sterilization (water is added)
  • 121 °C, 15 psi, 15 minutes to 20 minutes
  • Autoclaves and pressure cookers
25
Q

sterilization of Non-heat stable components

A

Filtration using 0.45 μm or 0.2 μm pore size filters such nitrocellulose and PTFE

26
Q

Examples of Non-heat stable components

A

organic acids, vitamins, antibiotics

27
Q

Included in the sterilization process to ensure that the sterilization is effective

A

Sterility Indicators

28
Q

ink/chemical that changes color due to high temp

A

steam autoclave tape

29
Q

tubes that contains heat resistant endospores , usually ____, which from purple turns to yellow

A

B. stearothermophilus

30
Q

For components that were filter sterilized they should be added prior to dispensing and when the media is cool enough. What is the temperature?

A

(around 50 °C).

31
Q

For blood agar plate, ______ of non-hemolyzed sheep or horse blood is added to cooled (50 °C) basal media.

A

5 to 10%

32
Q

the media should be heated again after adding the blood. The heating lyses the blood cells

A

chocolate agar

LBYBI13 (10 decks)

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