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cultivo de tejidos > Media types > Flashcards

Media types Flashcards

1
Q

Composition of basal media

A

Essential elements
carbon source
vitamins

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2
Q

Inorganic compounds

A

Macrnutrients : NO3, NH4, PO4, K, Ca2+, Mg2+

Micronutrientes: FE, CU, ZN, Mn, Mo, Co, I

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3
Q

Carbohydrates

A

Sucrose, Glucose & Myoinositol

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4
Q

Vitamins

A

Thiamine
pyridoxine
nicotic acid
biotin

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5
Q

amino acids

A

glycine

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6
Q

phytohormones

A

auxins
cytokinins
Gibberellins
Abscisic acid

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7
Q

Growth regulators

A

Auxins (NAA): Division an cell elongations, embryogenesis

Cytokinins (BAP): Cellular division, organogenesis, slows aging

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8
Q

Two hormones affect plant differentiation

A

Cytokinin: shoot development
Auxins: Root development

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9
Q

Other growth regulators

A

Gibberellins (GAs): Plant regeneration, germination
Abscisic acid (ABA): Evironmental stress
Ethylene: Inhibits cell division, DNA synthesis and growth meristems, Promotes fruit ripening

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10
Q

Main steps for micropropagation

A

Establishment
proliferation
rooting
hardening off

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11
Q

Fast and efficient way to get many new plants, disease-free, efficient, natual process

A

Micropropagation

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12
Q

Estabilishment

A

Selection of elite mother plant
Removal of explant (meristem, leaf, stem, bud)
Disinfection
Establishment

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13
Q

Proliferation

A

Transfer to proliferation media

Formation of stems (cytokinin:auxin ration of 50:1 produces shoots)

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14
Q

Rooting

A

Transfer plantlets to fresh medium

gradual strengthening of root system (addition of extra auxin.

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15
Q

Hardening off

A

Transfer in vitro plantlets

Pots of field

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16
Q

Methods of propagation

A

Organized–> Organ culture, embryo culture
Unorganized –> callus culture, cell culture in suspension
protoplasts.

17
Q

Tissue hyperhydricity

A

Excessive hydration
low lignification
impaired stomatal function
reduced mechanical strength

18
Q

Phenolization

A

Blackening and browning of callus pr explants shortly after isolation
Poor shoot development

19
Q

Albinism

A

Partial or complete loss of chlorophyll pigments
incomplete differentiation of chloroplast membranes
reduction to complete loss of photosynthesis

cultivo de tejidos (15 decks)

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