MAJOR HISTOCOMPATIBILITY COMPLEX Flashcards
memorization
In tissue/organ transplant, the donor and the recipient should be _____ and _____ matched
ABO and HLA matched
Siblings has a _____% chance HLA match
Sibling: 50% chance HLA match
Parents has a ____% chance HLA match
Parents: 25% chance HLA match
Disease(s) associated in HLA-B27:
Ankylosing spondylitis/bamboo spine disease
Disease(s) associated in HLA-DR3:
- Systemic Lupus Erythematosus
- Diabetes Mellitus I
- Grave’s disease
- Addison’s disease
- Myasthenia Gravis
Disease(s) associated in HLA-DR4:
- Rheumatoid arthritis
- Diabetes Mellitus I
Disease(s) associated in HLA-B8:
- Myasthenia Gravis
- Addison’s disease
- Celiac disease (RECALL🌟)
HLA that has the strongest correlation; patients that have this HLA type are 90x more prone to the disease associated with this HLA:
HLA-B27: Ankylosing spondylitis/ bamboo spine disease
Where is the genetic coding of HLA located?
Chromosome 6 - HLA is coded in chromosome 6
Class I genes:
HLA-A
HLA-B
HLA-C
Class II genes:
HLA-D
HLA-DP
HLA-DQ
HLA-DR
Class III genes:
C2
C4
Factor B
TNF
Location of Class I MHC
All nucleated cells
Location of Class II MHC
APCs (Macrophage, monocyte, B cells, dendritic cells)
NOTE: APCs also has class I MHC, but class II MHC is unique to APCs
Function of class I MHC
- Present Ag to CD8+ cells (T cytotoxic cells)
- Destruction of virally infected cells, tumor cells, graft rejection
Function of class II MHC
Presentation of Ag to CD4+ cells (T helper cells)
(Tissue matching technique) The donor and recipient cells are reacted with HLA Abs; simple and fast
Serological tissue matching method
What type of tissue matching method is MIXED LYMPHOCYTE REACTION?
Cellular tissue matching method
In Mixed Lymphocyte Reaction, it is used to used as an irradiator to inactivate donor lymphocytes:
MITOMYCIN C
In Mixed Lymphocyte Reaction, the amount of proliferation is measured using:
Thymidine [^3H] (Tritium labeled thymidine)
Mixed Lymphocyte Reaction is best for:
Bone marrow grafts and living-related donors
MLR only tests for what class of HLA?
HLA-D (Class II)
What type of tissue matching method is RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP)
Molecular tissue matching method
In RFLP, DNA sequences are amplified using ______:
Polymerase Chain Reaction
Which tissue matching method is the fastest and superior?
A. Serological
B. Cellular (MLR)
C. Molecular (RFLP)
C. Molecular (RFLP)
Which of the following is a tissue typing method test?
A. MLR
B. RFLP
C. CDC
C. CDC
NOTE: CDC or Complement Dependent Lymphocytotoxicity (Other name)
Complement Mediated MICROLYMPHOCYTOTOXICITY (original)
they are all the same, the CDC is the other name for Complement Mediated Microlymphocytotoxicity
What type of blood specimen is used in CDC?
ACD or Heparinized blood
In CDC tissue typing method, purified lymphocyte suspension is obtained by what technique?
Ficoll-Hypaque Centrifugation
In CDC, what will happen to the cells if the HLA antigen specific to the HLA antibody is present?
The complement is activated and kills the cell (lysed)
In CDC, what stain is used to visualize the cells after incubating with HLA Abs and complement?
Eosin Y or Trypan blue
What type of microscope is used in the CDC?
Inverted phase Contrast Microscope
Uninjured cells visualized in IPCM appear to be:
A. Flattened, large, dark, and non-refractile
B. Small, dark, and non-refractile
C. Small, bright, and refractile
D. flattened, large, bright, and refractile
C. Small, bright, and refractile
This indicates that the cells are negative for the HLA Ag
Injured cells visualized in IPCM appear to be:
A. Flattened, large, dark and non-refractile
B. Small, dark, and non-refractile
C. Small, bright, and refractile
D. flattened, large, bright, and refractile
A. Flattened, large, dark, and non-refractile
This indicates that the cells are positive for the HLA Ag
HLA-DR (class II) typing requires identification of:
B cell lymphocytes
B-lymphocyte suspension can be prepared by:
Nylon wool separation
