Lower GI Virus

Question 1

Rotavirus classification tree

Answer 1

RNA virus
Icosahedral nucleocapsid
Nonenveloped
DS segmented (class III)

Question 2

Where does rotavirus initially attach?

Answer 2

Absorptive villous epithelium of upper two-thirds of small intestine

Question 3

Which is more indicated in infantile gastroenteritis?

Answer 3

Rotavirus (50% of diarrhea hospitalization with infants)

Question 4

What is the viral toxin released by rotavirus and what does it do?

Answer 4

NSP4

Lodges into interface between ER interface and cytoplasm to disrupt Ca flux, killing the cell

Question 5

8 hours after rotavirus infections, what can you see on histological examination?

Answer 5

Cytoplasmic inclusion bodies

Question 6

What vitamin can you give to help with rotavirus infections in malnourished parts of the world?

Answer 6

Vitamin A

Question 7

What is the difference between the two rotavirus vaccines?

Answer 7

One is a human strain that’s been attenuated

The other is a hybrid between human and cow strain

Question 8

Norwalk virus classification tree

Answer 8

RNA virus
Icosahedral nucleocapsid
Nonenveloped
SS+ nonsegmented genome (Class IV)

Question 9

If there is a high fever and a viral illness is expected, which virus is more likely?

Answer 9

Norovirus

Question 10

Which viral infection sees more vomiting?

Answer 10

Norovirus

Question 11

What aids in antibodies not being super effective against future norovirus infections?

Answer 11

Genetic drift (High error rate with RNA polymerase)
So many viruses out there

Question 12

What is usually done to test for norovirus infection?

Answer 12

Not usually tested.

If tested, then RTPCR, electron microscopy, ELISA

Question 13

What is used to treat norovirus?

Answer 13

Peptobismol to relieve symptoms

Question 14

Adenovirus classification tree:

Answer 14

DNA virus
Icosahedral
Nonenveloped
DS DNA linear (Class I)

Question 15

If testing for adenovirus, what is usually done?

Answer 15

ELISA

PCR/DNA probe analysis