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BIOE 367 > LNP Delivery Systems > Flashcards

LNP Delivery Systems Flashcards

1
Q

The first approved lipid-based nanocarrier formulation was ____ in _____

A

Doxorubicin
1995

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2
Q

Components of an LNP

A
  • Cholesterol
  • PEG lipids
  • Helper phospholipids
  • Cationic ionizable lipids
  • mRNA
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3
Q

Ionizable lipids are ______ at a neutral pH and ______ at an endosomal pH

A

Uncharged
positively

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4
Q

ionizable lipids drive _____ of negatively charged payloads

A

encapsulation

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5
Q

Ionizable lipids are FUSOGENIC which means they are

A

capable of transitory interaction with lipid membranes

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6
Q

How is the zeta potential of LNP’s measured?

A

Folded Capillary Cell

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7
Q

pKa of ionizable lipids drive _____

A

endosomal escape

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8
Q

Cholesterol contributes to the ______ of LNP bilayers

A

Rigidity and stability

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9
Q

PEG lipid improves _____ of nanoparticle formulation by providing ________

A

physical stability
a steric barrier to particle aggregation

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10
Q

Which particle modulates pharmacokinetics and biodistribution in vivo?

A

PEG lipids

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11
Q

PEG lipids influence cell uptake and endosomal escape which means it impacts ____

A

interactions with target cells

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12
Q

Helper phospholipid is a bilayer-forming lipid which means it modulates

A

physical and biological properties of the nanoparticle lipid phase

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13
Q

Helper phospholipids also impact _____ and interactions with _____

A

particle stability
biological membranes

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14
Q

Techniques for LNP formulation

A
  • Microfluidic mixing
  • T-mix/ Vortex Mixing
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15
Q

What is microfluidic mixing?

A

a technique for LNP formulation that entails laminar chaotropic flow, reliant on dean vortices for mixing

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16
Q

what is Vortex mixing?

A

technique for LNP formulation that entails turbulent flow, reliant on turbulent eddys for mixing

17
Q

Nanoparticle properties influence the formation of ____

A

protein corona

18
Q

Encapsulation effeciency is measure by administering RiboGreen dye to measure ____

A

the ratio of mRNA in the cell to outside of the cell

BIOE 367 (10 decks)

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