Liver Function Test (ALT | P)

Question 1

What is the meaning of ALT?

Answer 1

Alanine Transaminase (Alanine Aminotransferase)

Question 2

What is the meaning of SGPT?

Answer 2

Serum Glutamic Pyruvic Transaminase

Question 3

What is ALT?

Answer 3

It is a cytosolic enzyme that is found in high concentrations in the liver

Question 4

What is the characteristic of ALT?

Answer 4

It is a liver-specific enzyme

Question 5

What is hepatocellular injury?

Answer 5

It is the trigger for the release of these enzymes into the circulation

Question 6

What are the diagnostic significance of ALT?

Answer 6

1) For the evaluation of hepatic disorder
2) Intrahepatic > extrahepatic (source)
3) It has a longer half-life compared to AST
4) ALT can be used to screen blood donors
5) ALT is a more sp and sensitive screening test for post-transfusion hepatitis

Question 7

What is the method present for ALT?

Answer 7

Enzymatic method

Question 8

What is the principle of rxn of enzymatic method?

Answer 8

L- Alanine + alpha(sign)-ketoglutarate (via the action of ALT) -> Pyruvate + L- glutamate

Pyruvate + NADH + H^+ (via the action of LDH) -> L- lactate + NAD^+

Question 9

What are the sources of error for ALT?

Answer 9

1) It is relatively unaffected by hemolysis

2) Stability: 3 - 4 days at ref temp

Question 10

What are the tubes present for the determination of ALT lvls?

Answer 10

1) ALT + Blank
2) ALT + Standard
3) ALT + Unknown

Question 11

What is the process (or steps) for ALT?

Answer 11

1) Label test tubes (blank, standard, unknown)
2) Transfer 1,000 uL of ALT working rgnt to each tube and place in a 37 DC H2O bath
3) Add 100 uL sx
4) Read and record absorbance at 5 mins using 340 nm

Question 12

What is the normal reference range of ALT?

Answer 12

3 - 35 IU/L