Liver Function Test (ACP | P)

Question 1

What is the meaning of ACP?

Answer 1

Acid Phosphatase

Question 2

What are the functions of ACP?

Answer 2

1) To diagnose the prostatic carcinoma
2) It is a more accurate in the dx of advanced prostatic cancer than the early dx
3) It can be used / done on vaginal swabs in rape cases

Question 3

Why can ACP be used on vaginal swabs in rape cases?

Answer 3

Because the seminal fluid is rich in acid phosphatase

Question 4

What are the tissue sources of ACP?

Answer 4

1) Prostate
2) Other sources: bone, liver, spleen, kidneys, RBCs, and bone marrow
3) Rare: breast carcinoma

Question 5

What are the sx considerations for ACP determination?

Answer 5

1) ACP is unstable if the pH is 7.0 >
2) Rectal examination, prostatic massage, urinary catheterization, or instrumentation of the prostate in the previous 2 days before the test may give false high values

Question 6

What is the principle of rxn for the method for ACP?

Answer 6

Alpha(sign) Naphthylphosphate + H2O (via the action of ACP) -> alpha(sign) Naphthol + Phosphate

Alpha(sign) Naphthol + Fast Red TR Salt -> Diazo dye complex

Question 7

What should be done to the ACP working rgnt?

Answer 7

It should be reconstituted

Question 8

What are the components of the ACP working rgnt?

Answer 8

1) Acid phosphatase rgnt
2) L-Tartrate rgnt
3) Acetate Buffer

Question 9

What are the components of ACP rgnt?

Answer 9

1) alpha(sign)-Naphthylphosphate
2) Na citrate
3) pH

Question 10

What are the concentrations / values of the ff components of ACP rgnt (in case of reconstituted rgnt):

1) alpha(sign)-Naphthylphosphate
2) Na citrate
3) pH

Answer 10

1) 3 mM
2) 60 mM
3) 5.3 +/- 0.1

Question 11

What are the components of L-Tartrate rgnt (in case of reconstituted rgnt)?

Answer 11

1) Sodium L-Tartrate
2) Citric acid
3) Na citrate
4) pH

Question 12

What are the concentrations / values of the ff components of L-Tartrate rgnt (in case of reconstituted rgnt):

1) Sodium L-Tartrate
2) Citric acid
3) Na citrate
4) pH

Answer 12

1) 2 M
2) 70 mM
3) 10 mM
4) 5.3 +/- 0.1

Question 13

What is the concentration of acetate buffer?

Answer 13

5 M

Question 14

What is the component of acetate buffer?

Answer 14

pH

Question 15

What is the value of pH (as a component of acetate buffer)?

Answer 15

5.0

Question 16

What is the process (or steps) for total ACP determination?

Answer 16

1) Label test tubes (blank, control, and unknown)
2) Pipet 1,000 uL ACP rgnt
3) Add 10 uL L-tartrate to all tubes
4) Add 100 uL sx to all tubes
5) Incubate for 5 mins
6) After 5 mins, read and record A at 405 nm

Question 17

What is the normal reference range for normal total ACP?

Answer 17

0 - 3 U/L

Question 18

What are the causes of increased concentration of serum ACP values?

Answer 18

1) Metastasizing carcinoma of prostate
2) Bone disease (Paget’s disease or female breast cancer that has metastasized to bone)
3) After prostate massage

Question 19

What is the cause of decreased concentration of serum ACP values?

Answer 19

No clinical significance to low concentration of serum ACP