Lipase (M)

Question 1

What is the meaning of LPS?

Answer 1

Lipase

Question 2

What is LPS?

Answer 2

It is an enzyme that hydrolyzes fats

Question 3

What is the difference between LPS and AMS?

Answer 3

LPS is more sp > AMS

Question 4

What is the fxn of LPS?

Answer 4

It is used to diagnose acute pancreatitis

Question 5

• If the pt has acute pancreatitis, what happens to the pt’s LPS lvls in the ff conditions?
  1) When does it rise?
  2) When does it remain elevated?

Answer 5

1) LPS lvls rise 4 - 8 hrs

2) LPS lvls remains elevated for 8 - 14 days

Question 6

What are the tissue sources of LPS?

Answer 6

1) Pancreas
2) Stomach
3) Small intestine

Question 7

What is the principle employed for determination of LPS lvls?

Answer 7

Turbidimetric

Question 8

What is the principle of rxn for determination of LPS lvls?

Answer 8

Triglyceride + H2O -> mono. + di-glycerides + fatty acids

substrate: 0.8% (w/v) olive oil in alcohol

Question 9

What is the process (or steps) for determination of LPS lvls?

Answer 9

1) Label 3 test tubes: BLANK, STANDARD, UNKNOWN
2) Place 3,000 uL of LPS working rgnt
3) Pipette 100 uL sx
4) Incubate for 5 mins
5) Read and record A at 400 nm

Question 10

What is the normal reference range of LPS lvls for adults?

Answer 10

10 - 150 U/L

Question 11

What is the normal reference range of LPS lvls for old individuals (60 yo >)?

Answer 11

18 - 180 U/L