Chloride (M)

Question 1

What is the chemical symbol for chloride?

Answer 1

Cl-

Question 2

What is the characteristic of Cl?

Answer 2

It is the most abundant extracellular anion

Question 3

What are the fxns of Cl?

Answer 3

It maintains:

1) Osmolality
2) Blood volume
3) Electric neutrality

Question 4

What are the conditions of abnormal lvls of Cl?

Answer 4

1) Hypochloremia

2) Hyperchloremia

Question 5

What are the sx considerations for Cl?

Answer 5

1) Serum, heparinized plasma, and urine can be used

2) Hemolysis causes dilutional effect (decreased lvls)

Question 6

What is the test principle for Cl (/ for Cl determination)?

Answer 6

Hg (SCN)2 + 2CI^- -> HgCI2 + 2SCN^-

3SCN^- + Fe^3+ -> 4 Fe(SCN)3 red complex

Question 7

What is the method (/ steps) for Cl determination?

Answer 7

1) Label test tubes
a. Blank (/ B)
b. Standard (/ S)
c. Unknown (/ U)
2) Pipette 1,500 uL chloride rgnt to all tubes
3) Add 10 uL of sx to respective tubes and mix
4) Incubate for 5 mins
5) Set the spectrophotometer at 480 nm (semi-automated analyzer can also be used)

Question 8

What is the normal reference range for Cl?

Answer 8

98 - 106 mEq/L