Lipid 1 Flashcards
dynamic flexible barrier
PM
ions sugars
other nutrients
active transport mechanism
ER
ass with enzymes
macromol transport
LDL (into cell)
collagen out of cell
non covalent intractions
membrane architecture
Carbohydrates ass with
lipids(glycolipids) or proteins(glycoproteins)
(on PM)
glycerol based lipids
PI PC PE PS
in the membrane
meyelin
higher lipid; protein
inner mit. membrane
lower lipid; protein
erthrocyte
middle lipid to protein compostion
insultating membrane
more lipids
very active membrane
more proteins
inner mit membrane
phospholipids and chol make up lipid in
erythrocyte
tf glycerol based lipids make up a huge percent of phospholipids
T
P…CES
make up 75%
tf in erythrocyte membrane , phospholipids are made up of
50% choline containing lipids
PC and SM
choline containing lipids
FA making up phospholipid
50% sat; 50 % unsat
20 diff proteins and complex
erythrocyte membrane
ret. rod protein
1protein and simple
rhodopsin
Rod proteins
water insol
oil sol
phobic
Fa chain on phospholipid
ampipathic
dual characteristics
phillic
water soluble
and oil insoluble
PC
– sealed, impermeable to polar molecules
vesicle(phospolipid bilayer strucuture)
inc C number
inc temp
DB are
cis in phospholipid bilayer
db induces kink and
lower temp
cholesterol
modulator of fluidity
cholesterol
OH at 3 position
ampipathic
SM and PC
outside of lipid membrane
SM and PC
choline containing
PE and PS
on inside of cell membrane
Membrane
preferential and assymetric distribution of lipoproteins
has a - charge with it
PS
(used for protein interactions)
used for signal event in cell
PE
very fast
Lat diffusion
flippase
for transverse diffusion
very slow
Need detergents to isolate
Integral/intrinsic membrane proteins
Integral/intrinsic membrane proteins
pump or transporter
Integral/intrinsic membrane proteins
Big protein icebergs floating in the 2D bilayer
pumps receptors enzymes
integral/intrinsic membrane proteins
Need pH and ionic strength to isolate these
Extrinsic membrane protein
attachments to the inner surface phospholipid heads
extrinsic proteins
structural to form the cell cytoskeleton
Enzymes recruited to the cytoplasmic side of the membrane to carry out a process
Extrinsic membrane proteins
erythrocyte
biconcave shape
osmotic shock
Hb out
membrane
red ghost
pure preparation of just the plasma membrane
tf red ghost is still biconcave because underneath the red cell membrane, there is a meshwork of proteins (cytoskeleton)
Red cell membrane ghost is still biconcave because underneath the red cell membrane, there is a meshwork of proteins (cytoskeleton)
detergent extraction
removes ghost
but biconcave cytoskeleton still there
