Light Microscope Flashcards
1
Q
Upnight vs inverted microscope
A
Upright= lightbelowstage
Inverted= light above stage
2
Q
Types of light microscope
A
Brightfield microscope
3
Q
Magnification
A
Enlarging object relative to original size.
Multiply magnifications
4
Q
Low-mid vs high power magnification
A
Low-mid: useful for scanning broad aspects
High: useful for increased detail, but lose appreciation of some areas of specimen
5
Q
Resolution
A
Ability to separate & distinguish 2 individual points in a specimen
“Sharpness” of image
Rule: cannot distinguish objects smaller than 1/2 the wavelength of lightsource
6
Q
Preparing samples
A
Fixing tissue/cells
Embedding
Sectioning
Staining
