Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Bacteriology Lecture > Lesson 11: Pseudomonas, Vibrio, Haemophilus > Flashcards

Lesson 11: Pseudomonas, Vibrio, Haemophilus Flashcards

1
Q

It is an opportunistic pathogen that infects burns, wounds, surgical incisions, and sites of catheterization

Most common cause of infections of burn injuries and outer ear (otitis externa)

A

Pseudomonas aeruginosa

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Is P. aeruginosa motile?

A

Yes, it is a flagellated bacterium with a polar flagellum, arranged in an amphitrichous manner

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

P. aeruginosa is non-capsulated but some strains posses a _________

A

slime layer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Is P. aeruginosa gram positive or negative?

A

Gram negative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

2 commonly used media for the cultivation of P. aeruginosa

A

Nutrient Agar Medium
MacConkey Agar Medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Optimum pH of P. aeruginosa

A

5.6 - 9.0 = can survive
6.6 - 7.6 = maximum growth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Some strains of P. aeruginosa show what type of hemolysis?

A

Beta hemolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

P. aeruginosa is an obligate aerobe meaning?

A

It can only grow in the presence of oxygen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What media is used for P. aeruginosa when the growth of the bacterium occurs as “dense turbidity” in the broth medium with a “surface pellicle”?

A

Trypticase Soy Broth
Nutrient Broth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

4 Types of Pigments of P. aeruginosa

A

Pyocyanin
Fluorescein
Pyorubicin
Pyomelanin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

In Nutrient Agar Medium, Pseudomonas aeruginosa produces what type of odor?

A

Sweetish odor
Musty or Earthy smell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

It is a selective medium for P. aeruginosa and other species of Pseudomonas which consists of “Cetrimide” that inhibits the growth of gram-positive bacteria and normal flora

A

Cetrimide Agar Medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Vibrio cholera on Monsur’s Gelatin Taurocholate Trypticase Tellurite Agar Medium

A

24 hours incubation:
- small colonies
- 1-2 mm in size
- grayish color with black centers

48 hours of incubation:
- increase to 3-4mm in size

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is the expected color in Cetrimide Agar Medium if there is presence of P. aeruginosa?

A

Greenish-blue coloration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

In MacConkey Agar Medium, P. aeruginosa shows what color since it is a non-lactose fermenter unlike E. coli and Klebsiella which are lactose fermenters?

A

None, since it is a non-lactose fermenter

Klebsiella and E. coli = Pink colony

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

He isolated “vibrions” from cholera patients because of their motility

A

Filippo Pacini

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Identify the name of the Pigment

Bluish-Green

A

Pyocyanin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Identify the name of the Pigment

Greenish-Yellow

A

Fluorescein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Rigid, curved rods, or “Comma shaped” and are highly motile-single polar flagellum

A

Vibrio

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Identify the name of the Pigment

Reddish-Brown

A

Pyorubicin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Identify the name of the Pigment

Brown to Black

A

Pyomelanin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

It is the most important pigment from the diagnostic point of view since it is not produced by any other species of Pseudomonas but only by the Pseudomonas aeruginosa

A

Pyocyanin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

It is characterized by sudden effortless vomiting, profuse rice water stool followed by rapid dehydration

A

Cholera

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

The motility of Vibrio cholerae is called as?

A

Darting motility (swarm of gnats)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Most medically important member of the genus Vibrio

A

Vibrio cholerae

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Vibrio cholerae was first isolated by him from cholera patients in Egypt

A

Robert Koch

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Growth of Vibrio cholerae is better on acidic or alkaline medium?

A

Alkaline medium (6.4 -9.6) (optimum 8.2)

15
Q

Vibrio cholera on Gelatin Stab Culture

A

After 3 days of incubation, a “White Line” of growth appears

15
Q

Selective Medium used for Vibrio cholera

Yellow colony
Sucrose fermentation
Readily visible against a dark green background of the agar

A

Thiosulphate citrate bile sucrose (TCBS) agar

15
Q

An Enrichment Medium used for Vibrio cholera

Rapid growth: 6 hours
Formation of Thick Surface Pellicle
1mL stool/10mL alkaline peptone water
37C for 4-6 hours incubation

A

Alkaline Peptone Water (pH 8.5)

16
Q

Vibrio cholera ferments what carbohydrates?

A

Glucose
Mannitol
Maltose
Mannose
Sucrose

16
Q

True or False

Vibrio cholerae are non-halophilic

A

True, they cannot grow in media with more than 6% sodium chloride

17
Q

Vibrio cholera on Nutrient Agar

A

Round
Moist
Translucent
“Bluish” colonies
1-2mm size

17
Q

Vibrio cholera on Blood Agar

A

A zone of “green discoloration” and will become “clear”

17
Q

Vibrio cholera on MacConkey Agar

A

Colorless colony, but will change to pink after a while

18
Q

Vibrio cholera on Alkaline Bile Salt Agar

A

Similar colonies on Nutrient Agar Medium since it is just a modified version

19
Q

Vibrio cholera does not ferment what carbohydrates?

A

Inositol
Arabinose
Lactose

20
Q

True or False

Carbohydrate metabolism is fermentive, producing acid, but no gas

A

True

21
Q

Vibrio cholera is:

Catalase and oxidase: positive
Nitrate Reduction: positive
Indole: positive

Methyl Red: Negative
Urease: Negative

A

True

22
Q

What test is used to identify V. cholera colonies and performed when a loopful of growth is mixed with a drop of 0.5% sodium deoxycholate on a slide.

Mixture loses turbidity: mucoid and forms a “string” when loop is drawn away slowly = positive test

A

String Test

23
Q

Route of Transmission of Vibrio cholera

A

Fecal-Oral route

24
Q

Infectious dose of cholera in WATER

A

10^9

25
Q

Infectious does of Cholera in FOOD

A

10^3

26
Q

Incubation period of cholera

A

1 to 4 days

27
Q

Assists the enterotoxin of vibrio cholera as it serves as a mucosal receptor for subunit B, which promotes entry of subunit A into the cell

A

Ganglioside GM1

27
Q

Activation of this subunit of the enterotoxin of Vibrio cholera yields increased levels of intracellular cAMP and results in prolonged “hypersecretion” of water and electrolytes

A

Activation of subunit A1

28
Q

This toxin of cholera binds to cell membrane via B sub-unit and this reverts back to allow A sub-unit to enter the cell membrane

A

Cholera Toxin
Cholera Enterotoxin
Choleragen

28
Q

Habitat of Haemophilus

A

Upper Respiratory Tract of Humans (Nasopharynx of approximately 75% of healthy people)

29
Q

Can H. influenza growth in a candle jar with 5% CO2?

A

Yes

29
Q

This occurs when the colon cannot absorb the fluid fast enough. These factors contribute to decrease blood pressure.

A

Diarrhea

29
Q

“Vibrio cholerae” colony color on TCBS (Thiosulfate Citrate Bile Salt Sucrose) Agar

A

Yellow

30
Q

“Vibrio parahaemolyticus and Vibrio vulnificus” colony color on TCBS (Thiosulfate Citrate Bile Salt Sucrose) Agar

A

Green

30
Q

Identify the bacteria:

Small
Pleomorphic
Gram-negative bacilli or coccobacilli with random arrangements
Found on mucous membrane of the URT

A

Haemophilus influenza

30
Q

True or False

Encapsulated strains appear more mucoidal than non-encapsulated strains, which appear as smaller, compact grey colonies for H. influenza

A

True

30
Q

True or False

H. influenza required hemin (X factor) and NAD (V factor) for growth

A

True

31
Q

Standard Medium used for the growth of H. influenza

A

Chocolate Agar Plate (CAP)

prepared with: heat-lysed horse blood( a good source of hemin and NAD) ; can also use sheep blood

31
Q

Characteristic of H. influenza on CAP

A

Large
Round
Smooth
Convex
“Colorless to grey”
Opaque

32
Q

What is the smell of H. influenza on plates?

A

Pungent indole smell

33
Q

What agar is used for H. influenza that shows a characteristic:

“Flat pinpoint” colony
Shows “satellitism” with S. aureus

A

Blood Agar

34
Q

What type of hemolysis does H. influenza show on Horse Blood Agar?

A

Beta-hemolysis

35
Q

What type of H. influenza colonizes the nasopharynx, and may penetrate the epithelium and capillary endothelium to cause “bacteremia”

A

Type b H. influenza

36
Q

This type of H. influenza can cause meningitis, epiglottitis, bacteremia, and cellulitis

A

Type b H. influenza

37
Q

This type of H. influenza can cause otitis media, sinusitis, tracheobronchitis, and pneumonia

A

Nontypable H. influenza

38
Q

Haemophilus species that causes “pneumonia and endocarditis”

A

H. parainfluenza

39
Q

Haemophilus species that causes “genital chancre”

A

H. ducreyi

40
Q

Haemophilus species that causes “conjunctivitis or Brazilian purpuric fever”

A

H. aegyptius

Bacteriology Lecture (11 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions