Lecture 5-Endo/exocytosis Flashcards
Transport vesicles are coated with either _____ or _____ protein
clatharin
coat
_____ mediates transport of vesicles from the golgi network and plasma membrane
clathrin
Coat protein coated vesicles transport between the ____ and ______
rER, golgi
_____ contain acid hydrolases and degrade proteins, nuclei acids, phospholipids, and oligosaccharides
Lysosomes
______ _ ______ gets added to proteins that need to be lysed
Mannose 6 phosphate
binding of clatharin to a vesicle is mediated by proteins called _____
adaptins
_____ are formed by endocytosis from plasma membrane that usually fuse with lysosomes
endosomes
_____ is “cell drinking” via small vesicles called caveolae
pinocytosis
The uptake of fluid via pinocytosis is mediated by caveolae vesicles coated with ____
caveolin
_____ is the ingestion and degradation of foreign material taken into the cell by RME
Heterophagy
______ involves the segregation of an organelle within membranes from the rER to form a vacuoule to be digested.
Autophagy
Receptor mediated endocytosis involves ____ coated pits that have a ____ appearance in microscopy
clatharin
fuzzy
_____ _____ _____ is the specific uptake of a substance such as LDL by a cell with plasma-membrane receptors for that substance
receptor mediated endocytosis
What process follows this manner?
- Ligand binds receptor
- Membrane invaginationand endosome formation
- Proton pump acidifies endosomal compartment
- Endosome moves along MT tracks towards Trans golgi reticulum
- Interaction in golgi determines fate of ligand/receptor
receptor mediated endocytosis
_____ is a protein that forms clatharin
triskelion
An example of an RME fate where the ligand is degraded in the lysosome while the receptor is recycled is ____
LDL
Example involving RME where both the ligand and receptor are recycled:
ttransferrin
Example where ligand and receptor are both degraded in lysosomes:
epidermal growth factor
Endocytosis with a non-coated pit containing a cholesterol binding protein is mediated by _____
caveolin
_____ and tetanus toxin utilize a clatharin independent pathway
Cholera
Exocytosis requires the interaction of receptors in both the granule and plasma membrane, as well as the _____ of the two phospholipid membrane bilayers
coalescence
Exocytosis secretion can be either _____ (release of materials is due to extracellular signal) or _____ (no signal required)
regulated
constitutive
_____ are small vesicles secreted by cells that can be captured by other cells. Example is antigens being transferred between dendritic cells
Exosomes
Tay sachs, hurler’s syndrome, gaucher’s syndrome, and neiman-pick disease are all examples of _____ storage diseases
lysosomal
Lysosomal storage diseases are often involved in issues with ______ degradation
lipid
the ______ virus is due to endocytosis of an infections agent
influenza
_-cell disease is caused by the failure to tag the enzymes that are supposed to be transported from the golgi to the lysosomes with M6P
i
______ is caused by failure to synthesize an enzyme needed to break down proteoglycans
mucopolysaccharaidosis
Coat protein _ vesicles transfer between ER and golgi, while coat protein _ vesicles transfer between golgi stacks
2
1
_____ endosomes are more electron lucent and may fuse with other vesicles to form CURL (compartment of uncoupling of receptor and ligand)
early
_____ results from a defeciency in hexosaminidase and results in an accumulation of glycolipids in the nervous system
Tay-sachs
_____ syndrome is due to a deficiency in an enzyme that breaks down heparan and dermatan sulfate
Hurler’s
____ syndrome is due to defective glucocerbrosidase, causing glucocerebrosides to accumulate in the CNS and spleen
Gaucher’s
Neiman-pick disease is due to defective ______
sphingomyelinase
the ______-_____ virus binds to a receptor present on the surface of b-cells, causing mononucleosis and the development of lymphoma
ebstein-barr
