Lecture 5-Endo/exocytosis

Question 1

Transport vesicles are coated with either _____ or _____ protein

Answer 1

clatharin

coat

Question 2

_____ mediates transport of vesicles from the golgi network and plasma membrane

Answer 2

clathrin

Question 3

Coat protein coated vesicles transport between the ____ and ______

Answer 3

rER, golgi

Question 4

_____ contain acid hydrolases and degrade proteins, nuclei acids, phospholipids, and oligosaccharides

Answer 4

Lysosomes

Question 5

______ _ ______ gets added to proteins that need to be lysed

Answer 5

Mannose 6 phosphate

Question 6

binding of clatharin to a vesicle is mediated by proteins called _____

Answer 6

adaptins

Question 7

_____ are formed by endocytosis from plasma membrane that usually fuse with lysosomes

Answer 7

endosomes

Question 8

_____ is “cell drinking” via small vesicles called caveolae

Answer 8

pinocytosis

Question 9

The uptake of fluid via pinocytosis is mediated by caveolae vesicles coated with ____

Answer 9

caveolin

Question 10

_____ is the ingestion and degradation of foreign material taken into the cell by RME

Answer 10

Heterophagy

Question 11

______ involves the segregation of an organelle within membranes from the rER to form a vacuoule to be digested.

Answer 11

Autophagy

Question 12

Receptor mediated endocytosis involves ____ coated pits that have a ____ appearance in microscopy

Answer 12

clatharin

fuzzy

Question 13

_____ _____ _____ is the specific uptake of a substance such as LDL by a cell with plasma-membrane receptors for that substance

Answer 13

receptor mediated endocytosis

Question 14

What process follows this manner?

1. Ligand binds receptor
2. Membrane invaginationand endosome formation
3. Proton pump acidifies endosomal compartment
4. Endosome moves along MT tracks towards Trans golgi reticulum
5. Interaction in golgi determines fate of ligand/receptor

Answer 14

receptor mediated endocytosis

Question 15

_____ is a protein that forms clatharin

Answer 15

triskelion

Question 16

An example of an RME fate where the ligand is degraded in the lysosome while the receptor is recycled is ____

Answer 16

LDL

Question 17

Example involving RME where both the ligand and receptor are recycled:

Answer 17

ttransferrin

Question 18

Example where ligand and receptor are both degraded in lysosomes:

Answer 18

epidermal growth factor

Question 19

Endocytosis with a non-coated pit containing a cholesterol binding protein is mediated by _____

Answer 19

caveolin

Question 20

_____ and tetanus toxin utilize a clatharin independent pathway

Answer 20

Cholera

Question 21

Exocytosis requires the interaction of receptors in both the granule and plasma membrane, as well as the _____ of the two phospholipid membrane bilayers

Answer 21

coalescence

Question 22

Exocytosis secretion can be either _____ (release of materials is due to extracellular signal) or _____ (no signal required)

Answer 22

regulated

constitutive

Question 23

_____ are small vesicles secreted by cells that can be captured by other cells. Example is antigens being transferred between dendritic cells

Answer 23

Exosomes

Question 24

Tay sachs, hurler’s syndrome, gaucher’s syndrome, and neiman-pick disease are all examples of _____ storage diseases

Answer 24

lysosomal

Question 25

Lysosomal storage diseases are often involved in issues with ______ degradation

Answer 25

lipid

Question 26

the ______ virus is due to endocytosis of an infections agent

Answer 26

influenza

Question 27

_-cell disease is caused by the failure to tag the enzymes that are supposed to be transported from the golgi to the lysosomes with M6P

Answer 27

i

Question 28

______ is caused by failure to synthesize an enzyme needed to break down proteoglycans

Answer 28

mucopolysaccharaidosis

Question 29

Coat protein _ vesicles transfer between ER and golgi, while coat protein _ vesicles transfer between golgi stacks

Answer 29

2

1

Question 30

_____ endosomes are more electron lucent and may fuse with other vesicles to form CURL (compartment of uncoupling of receptor and ligand)

Answer 30

early

Question 31

_____ results from a defeciency in hexosaminidase and results in an accumulation of glycolipids in the nervous system

Answer 31

Tay-sachs

Question 32

_____ syndrome is due to a deficiency in an enzyme that breaks down heparan and dermatan sulfate

Answer 32

Hurler’s

Question 33

____ syndrome is due to defective glucocerbrosidase, causing glucocerebrosides to accumulate in the CNS and spleen

Answer 33

Gaucher’s

Question 34

Neiman-pick disease is due to defective ______

Answer 34

sphingomyelinase

Question 35

the ______-_____ virus binds to a receptor present on the surface of b-cells, causing mononucleosis and the development of lymphoma

Answer 35

ebstein-barr