Lecture 4 Viruses and other Acellular infectious agents Flashcards
Acellular Agents
viruses, Viroids, Satellites, Prions
Type of acellular agent that is protein and nucleic acid
Viruses
Type of Acellular agent that is only RNA
Viroids
Type of Acellular agent that is only nucleic acids
Satellites
Type of acellular agent that is only proteins
Prions
Viruses are a major cause of _____
disease
Viruses are important members of ____ world
aquatic
Are viruses important in evolution
yes
Viruses are important ____ systems in molecular biology
model
Bacterial viruses are called ______
bacteriophages (phages)
There are ___ archaeal viruses
few
Most viruses are ____ viruses
eukaryotic (plants, animals, protists, and fungi)
Viruses are classified into families based on
genome, structure, life cycle, morphology, genetic relatedness
a complete virus particle is called a ____
virion
General Properties of Viruses
- a complete virus particle
- consists of greater than or equal to 1 molecule of DNA or RNA enclosed in coat of protein
- May have additional layers
- cannot reproduce independent of living cells nor carry out cell division (but can exist extracellularly)
A virion size range is about ______ nm in diameter and most viruses must be viewed with an _____-
10-400 nanometers, electron microscope
All virions contain a _____ which is composed of ______ and a ______
nucleocapsid, which is composed of nucleic acid (DNA or RNA) and a protein coat (capsid)
What are the protein subunits of a virion capsid called
capsomeres
All Virion structure
- Nucleic Acid (DNA or RNA) (Single or double stranded)
- Capsid (Protein coat) (Protein subunits= Capsomeres)
All virions contain a nucleocapsid which is composed of ____ and a _____ but some consist only of a nucleocapsid, others have additional components called ____
Nucleic acid (DNA or RNA) and protein coat (capsid), Envelopes
The enveloped that surrounds the nucleocapsid of viruses usually has viral proteins called ____ inserted into it
spikes
What are the three types of capsid symmetry
- helical
- icosahedral
- complex
The envelope that surrounds some viruses is composed of
combination of lipids and carbohydrates , and proteins
Envelope lipids and carbohydrates are acquired from the ____. while envelope proteins are coded for by _____ and may even project form the envelope surface as ____
host, viral genes, spikes (also called peplomers)
What is the function of envelope spikes (peplomers)
attachement to host cell surface
General Characteristics of Viruses
- Obligatory intracellular parasites
- Contain DNA or RNA
- Contain a protein coat (note that the DNA/RNA and protein coat make up the nucleocapsid present in all viruses)
- Some are enclosed by an envelope
- Some viruses have spikes
- Most viruses infect only specific types of cells in one host
- Host range is determined by specific host attachment sites and cellular factors
Large macromolecular structures which serve as protein coat of virus
Capsids
Functions of virus capsid
Protect viral genetic material and aids in its transfer between host cells
Virus Capsids are made of protein subunits called
protomers or capsomeres
Viruses are classified by ___-
morphology
examples of viruses classified by morphology
- helical viruses
- polyhedral viruses
- Enveloped Viruses
- Complex viruses
Viruses shaped like hollow tubes with protein walls
Helical Capsids
In helical capsids ____ self assemble
protomers
In helical capsids the size of the capsid is a function of ______
nucleic acid
Examples of helical viruses
- Rabies
- Ebola hemorrhagic feber
a _____ is a regular polyhedron with 20 equilateral faces and 12 vertices
icosahedron
An icosahedron is a regular polyhedron with ____ equilateral faces and ___ vertices
20, 12
______ are the most efficient way to enclose a space
Icosahedral capsids
Icosahedral capsids are composed of ____, which are ring or knob-shaped units, each usually 5 or 6 protomers
Capsomers
5 subunit capsomers are called
Pentamers (pentons)
6 subunit capsomers are called
Hexamers (hexons)
Pentamers are usually at the ____ of the icosahedron, whereas hexameters generally form its ____ and ____
vertices, edges and triangular faces
Example of polyhedral virus
- Adenovirus
- Poliovirus
what is the largest animal virus
poxvirus
Viruses that do not fit into the category of helical or icohedral capsids are called
Capsids of complex symmetry
Examples of complex viruses
- Poxvirus
- Large bacteriophages
some Large bacteriophages have ____ symmetry
binal (head resembles icosahedral, tail is helical)
many viruses are bound by an outer, flexible, membranous layer called the ____
envelope
Animal virus envelopes (lipids and carbohydrates) usually arise from
host cell plasma or nuclear membranes
Examples of enveloped viruses
- Influenza virus
- Herpes simplex virus
- rabies
Envelope proteins, which are viral encoded, may project from the envelope surface as ____/____
spikes/peplomers
functions of envelope protein, spikes/peplomers?
- involved in viral attachment to host cell
- used for identification of virus
- may have enzymatic or other activity
- may play role in nucleic acid replication
The influenza virus has spikes that consist of enzyme ________, which functions in the release of mature virions from the host cell
Neuraminidase
The influenza virus has spikes that consist of _____ proteins, so named because they bind virions to red blood cells and cause the cells to clump together- a process called ______
hemagglutinin, hemagglutination
Do virions have a variety of enzymes
yes
Some other virus enzymes are associated with the ____ or ____ but most are within the _____
envelope or capsid, but most are within the capsid
Characteristics of the viral genome
- Diverse nature of genomes
- a virus may have single or double stranded DNA or RNA
- The length of the nucleic acid also varies form virus to virus
- Genomes can be segmented or circular
What is the first step in the life cycle of a virus
attachment (often called absorption)
Steps of animal viruses life cycle
- Attachement: viruses attach to cell membrane
- Penetration/Entry: Endocytosis or fusion
- Uncoating: viral or host enzymes
- Biosynthesis: Production of nucleic acid
- Maturation: Nucleic acid and capsid proteins assemble
- Release: by buddying (enveloped viruses) or rupture (lysis)
HIV binds to what two proteins on human cells
CD4 and CCR5
attachement (adsorption) of virus is ____ specific
receptor
The receptor determines host preference for attachment (adsorption) of virus: may be ______, _______, _____, ____
may be specific tissue, may be more than one host, may be more than one receptor, maybe in lipid rafts providing entry of virus
after attachment to the host cell, the virus’s ____ or the entire ____ enter the cytoplasm
genome or entire nucleocapsid
Viral entry and uncoating varies between ___ or ____ virus
naked or enveloped
What are the 3 methods of viral entry and uncoating
- fusion of the viral envelope with host membrane; nucleocapsid enters
- endocytosis in vesicle; endosome (and endosomal enzymes) aid in viral uncoating
- Injection of nucleic acid
RNA virus must carry in or synthesize the _____ proteins necessary to complete synthesis
proteins
___ proteins are important in the assembly phase of the virus life cycle
late