Lecture 4

Question 1

___ virus infection involves a complete cell interaction

Answer 1

lytic

Question 2

_____ infection results in the multiplication and release the virus

Answer 2

productive

Question 3

integration of viral genome or part of it into host and alter or transforms host cell. can result in cancer

Answer 3

transformation

Question 4

one step growth cycle

Answer 4

Attachemant/adsorption, penetration, eclipse, rise

Question 5

viral replication steps

Answer 5

attachment, penetration, uncoating, replication, assembly

Question 6

what causes the ionic attraction of the virus to the cell?

Answer 6

heparn sulfate

Question 7

what do viral peplomers, spikes, capsomers, and glycoproteins interact with?

Answer 7

cell receptors

Question 8

What makes adsorption being independent of temperature useful for?

Answer 8

can synchronize adsorption, so that you can look at mechanisms of penetration and possile inhibitors

Question 9

___ is amount of infectious virus adsorbed per cell

Answer 9

MOI, multiplicity of infection

Question 10

entry into cell can be determined by EM or by no longer being ably to ___ virus and make it noninfectious

Answer 10

neutralize

Question 11

antibody binding

Answer 11

neutralize

Question 12

Where are viruses adsorbed to

Answer 12

microvilli cell surfaces or to clathrin coated pits

Question 13

what do clathrin-coated vesicles in cytoplasm do?

Answer 13

fuse with endosomes for transport of virus to nucleus or uncaring in cytoplasm

Question 14

virus goes directly into cytoplasm by lipid to lipid fusion

Answer 14

membrane fusion

Question 15

____ in partially uncoated to subviral particles in cytoplasm protecting the nucleic acid

Answer 15

reovirus

Question 16

phase where infectious virus is not detected in cells

Answer 16

eclipse

Question 17

What kind of obstacles do this viruses have to overcome before uncoating?

Answer 17

antibodies, receptors, enzymatic degradation, nucelases,

Question 18

What are some of the structural components that viruses use from their host cells to replicate?

Answer 18

actin fibers, membranes, ribosomes

Question 19

How is the transcription regulated in the early period?

Answer 19

alteration of host NA, and protein synthesis by viral coded proteins

Question 20

What are the two parts of the matrix for virus replication?

Answer 20

virogenic stroma, cytoplasmic factories

Question 21

What is the virogenic stroma?

Answer 21

condensed chromatin region

Question 22

When does the late period of replication occur?

Answer 22

After the viral genome is made

Question 23

What happens in the late period?

Answer 23

structural proteins mostly made, and some are glycosylated

Question 24

____ _____ of polyproteins in the late period makes individual virus protteins

Answer 24

posttranslational cleavage

Question 25

What kind of polymerases and enzymes do DNA viruses use in late period?

Answer 25

viral or cellular

Question 26

Why is the late period complicated for RNA virus?

Answer 26

have to switch back and forth between the polarity of the RNA strands

Question 27

self assembly of capsids occurs even without nucleic acids

Answer 27

morphogenesis

Question 28

____ of cells is common for unenveloped virus release

Answer 28

lysis

Question 29

_____ occurs for enveloped virus release

Answer 29

budding