Exam 2 Flashcards
1
Q
How is papilloma viral DNA maintained?
A
staable multicopy plasmid that replicates once per cell cycle
2
Q
Which virus family can replicate either vegitvely or productively in differentiated cells
A
papilloma
3
Q
What are the two types of regulatory elemenrys for papilloma?
A
cis and tras
4
Q
How many promoters of transcription does papilloma have?
A
multiple
5
Q
Does papilloma splice?
A
yes - very complex
6
Q
What does the E1 do in the papilloma virus?
A
aids in plasma replication
7
Q
What does E6 and E7 due in the papilloma virus?
A
serve as human transformative genes
8
Q
Which strains of papilloma cause genital warts?
A
Strains 6 and 11
9
Q
Which strains of papilloma are the most prevalent ones to cause cancer of the cervis?
A
16 and 18
10
Q
Which papilloma virus causes penile cancer?
A
42
11
Q
How does E7 work in the papilloma virus?
A
Has transformative properties
transactivated Adenovisus E2 gene
interacts with pRB
12
Q
Which adenovirus gene is transactivated by papilloma E7?
A
E2
13
Q
How does E6 work in Papilloma virus?
A
-transmors keratinocytes to immortality by using ubiquitin to mark p53 for destruction
14
Q
Which virus causes the following diseases: warts, oncogenital condylomas, laryngeal papilomatosis, flat condelyomas, neoplasia of penis, anus, and cervix
A
papilloma
15
Q
How long is the eclipse period for polymer and papilla?
A
12-14 hr
16
Q
What does the polymer virus attach to on the cell?
A
Vp1 to cell glycerin growth factor receptor
17
Q
How does polymer enter the cell?
A
receptor mediated endocytosis
18
Q
Where does polyoma uncut?
A
in the nucleus
19
Q
What is transcribes as part of the early mRNA in polyoma?
A
T- antigens
20
Q
What is transcribed late in Polyoma?
A
virion proteins
21
Q
What is the structure of polyommar DNA?
A
covalently closed circle with no end
22
Q
What does polyooma use to open DNA?
A
primers
23
Q
What adds on primers to polyooma DNA?
A
DNA pol i
24
Q
What directions does polyooma synthesize in?
A
both forward and retro
25
What are the promoter elements of polyoma?
early tumor antigens - down regulate their own enhancer
26
How many promoters does polyooma have?
single
27
Does polyooma splice?
yes, not as complex as papilloma
28
What do the two directions of polymer transcription give?
T antigens and capsid proteins
29
Disease with progressive multifocal leukocephaly, demylenates CNS, latent in brain, and might be oncogenic
JC
30
What type of virus is JC?
polyoma
31
causes dysplastic growth in the urinary tract
BK
32
causes mild respiratory disease, there are antibodies in almost all adults
general polyoma
33
What are two types of JC virus?
BK and SV40
34
Sv40, BK, and JC are all types of
polyomaviridae
35
HPV types 1-80 are al types of
papillomaviridae
36
90% of cancer is caused by
oxidative stress, tobaccom and virus
37
What happens when p53 is destroyed?
cell replication levels increase, leading to cancer
38
What happens when pRB is inhibited?
tumor likelihood increases
39
Which virus has inverted complementary sequences?
adeno
40
Which aden virus has ends with different sequences?
autonomous virus
41
Which adeno virus is a helper viruse to herpes and AAV?
dependo
42
Which aden virus subfam has a bump in the DNA from the replicase to bind to?
autonomous
43
What does parvovirus bind to at the cell in order to attach?
heparan sulfate
44
Where does parvovirus replicate?
nucelus
45
Where does the AAV bind in order to enter the cell?
heparaan
46
Which virus integrates into chromosome 18 if no helper viruses are present?
AAV
47
What happens to AAV if adenovirus or herpes is there?
uses aden and herpes viruses for infection
48
Does splicing occur for the parvovirus?
yes
49
What is the replication model for parvovirus?
repeat DNA flips down to polymerize, no primer needed
50
What is the protein that packages parvo?
Ns1 - potential vaccine target
51
What ways can parvoviruses be spread?
respiratory tract, blood transfusion, across placenta
52
Which virus is associated with the following symptomsmild fever, headace, itching, rash, biphasic lacey rash after viremia, arthritis?
parvovirus
53
disease associated with slapped cheeks, miscarriages by hemolysis of fetal blood cells and cardiac failure
5th
54
5th disease is what type of virus
parvovirus
55
Associated with 4-5 days of viremia, rash comes after fever
b19
56
What kind of virus is b19
parvo
57
What is the incubation period for parvo
10 days
58
Where is parvo common?
daycares, 50% of adults have been exposed
59
how is parvo diagnosed?
electron microscopy, elisa, pcr
60
What virus may cause pneumonia?
bocavirus
61
is there a treatment for parvo
no
62
What does the adenovirus look like?
lots of knob proteins, fivers, and lots of hexons
63
What are the adenovirus groups based on?
serological reactions to the different fibers
64
What types of cells do adenoviruses infect?
adenoids, phargeal tonsils, palatine tonsils
65
How is adenovirus adsorbed?
fiber tail to receptor
66
When doe adenovirs penetrate
when pentane be binds to cell and integrates
67
Is adnovirus naked DNA infectious?
yes
68
This virus can inhibit cellular DNA proteins synthesis
adenoviruse
69
this virus has semiconservative DNA replication
adenocirus
70
what allows for the circularization of adenovirus?
terminal redundancy
71
What are the two shapes of DNA?
circular and linear
72
What forms the transforming region of adenovirus?
T antigen
73
what adenovirus transcript is needed to continue the rest of transcription
E1A
74
which adenovirus transcript blocks E2F to inactivate pRB (also disables p53)
e2
75
which virus causes the following symptoms: adenoids enlarged, acute respiratory disease, sore throat, pneumonia, tonsillitis, pink eye, pertussis like symptoms, gastroenteritis, rarely encephalitis,
adenovirus
76
Which adenovirus is associated with pneumonia + mortality in infants and elderly + acute reps disases
adv 7
77
which adenoviruses cause pink eye
3 7 14
78
how is adenovirus diagnosed?
swabs or cultures
79
What does CPE of adenovirus show?
round and clustering of cells, intranuclear inclusions,
80
Which adenoviruseses can be persistent for years?
1 2 5
81
How are adenoviruses transmitted?
fecal-oral, inhalation
82
Does herpes virus have an envelope?
yes
83
What shape is herpes?
icosahedral
84
are there enzymes in the herpes virion
no
85
what is different about herpes structure
amorphous tegument
86
why are herpes vaccines hard to make?
no common antigen
87
what is the shape of the herpes DNA
double stranded linear
88
is naked herpes DNA infectious?
yes
89
how is the herpes virus replicated?
rolling circle
90
What is the use of the herpes DNA packaging?
repeated sequences are used as bookends to decide which DNA parts ae packaged
91
what cleaves the DNA in herpes virus?
terminase - may be possible vaccine target
92
how does herpes attach to the cell
glycoproteins on viral envelop
93
how does herpes enter the cell
fusion with plasma cell membrane
94
what are the ways herpes penetrates?
either direct or RM end cytosine to PVRL1
95
where does herpes uncut at
nuclear pore, releases DNA in nuclus
96
blocking this intermediate early herpes gene inhibits translaion
IE3
97
these proteins are syntheszed by early herpes RNAs and then transcribed by cell to RNA pol II
alpha
98
a herpes transactivator proteins of alpha genes
aTIF
99
herepes gene that shutdown host proteins synthesis
VHS
100
herpes - these proteins are the structural and gyloproteisn
amma
101
does ran splicing occur
limited
102
how is the herepes envelop acquired?
inner nuclear membrane
103
how is herpes released>
reverse phagocytosis
104
what happens to cell when herpes released?
cel not killed but functions taken over
105
where are viral herpes genomes during latency
episomally, not integrated, also highly methylated
106
Burkitts lymphoma is associated with
EBV integrated or episomally
107
this virus causes cold cores, fevers, encephalitis, genital ingections
HSV1
108
where does HSV1 remain latent?
in the sensory ganglia trigemicnal nerve
109
this virus is sexually transmitted, occasionally oral lesions, 5+ recurrent infections a year
HSV2
110
where does the HSV2 remain latent
sacral plexus
111
chain terminating nucleotide used for herpesvirus
Acyclovir
112
this virus is respitaroly spread, results in rash or pox, infective for 2 weeks, more severe in adults
varicella-zoster
113
what is the vaccine for shingels
zostavax
114
this virus causes no obvious disease, but may result in miscarriages, transmitted by saliva milk and semen, problem for immunisupressed, and link to schizophrenia
Cytomeglavirus
115
primary infection caues mono, burkitts lymphoma in aid patients, people have very different reactions to it
EBV
116
the virus that causes roseola, possibly multiple sclerosis and possibly seizures
HHV6
117
the virus linked to copses sarcoma in Aid patients, and also linked to prostate cancer
HHV8
118
what is the overall structure of the pox virus
lateral bodies, 1, 2, or indistinct
119
are there enzymes in the pox virus
yes
120
is there an outer envelop for pox virus
yes
121
where does the pox virus attach
VGF protein
122
where does pox uncoat
cytoplasm
123
what is required for pox uncoating
RNA pol from virion
124
which small pox has the higher mortality rate
major
125
this virus causes rounded papillose that can be sexually transmitted, common in daycares, treated with liquid nitrogen
molluscum
126
yabapox comes from...
monkey
127
tanapox comes from...
insect bites
