Lecture 30 - Ox Phos Regulation Flashcards
ATP Synthase is also known as “_______.”
Complex V
The F1 subunit of ATP synthase is comprised of:
alpha 3, beta 3, gamma, delta, and epsilon subunits
Wha F1 subunits are arranged in a hexameric ring?
alpha and beta
The ___ subunit of the hexameric F1 ring is catalytically active.
beta
Above the alpha and beta subunit ring, there exists a “stalk” that connects the F1 to the F0 subunits. What comprises this “stalk?”
gamma and epsilon proteins
Each ____ subunit in the F1 region has a distinct region connected to the stalk.
beta
Conformation of the __ subunits occurs as H+ flow through the ATP synthase.
beta
What are the three steps of ATP synthesis in the enzyme ATP synthase?
Binding of ADP + Pi (L conformation)
ATP synthesis (T conformation)
Release of ATP (O conformation)
The “O conformation” of the beta subunit release the newly synthesized ATP. What co-factors is required for this release?
H+
The “proton conducting unit” of the ATP synthase is the (F1/F0) region.
F0
The F0 region is comprised of what subunits?
The ring c subunits (8-14) and an a subunit.
This enzyme moves newly synthesized ATP from the matrix to the cytosol A. ADP-ATP synthase B. ATP synthase C. Complex V D. ATP-ADP translocase
D. ATP-ADP translocase
This prevents the hydrolysis of ATP by the reverse reaction of ATP synthase.
Inhibitory factor 1
ATP synthase can be uncoupled from oxidative phosphorylation by these proteins.
UCP 1 through 3
Uncoupling of ox-phos occurs in the _______, which is rich in mitochondria.
A. Brown adipose tissue
B. White adipose tissue
C. Principle cells of the kidney
D. Central nervous system
A. Brown adipose tissue
Allows for the inhibition of ATP synthase by binding to c-subunit A. 2,4-DNP B. Atractyloside C. Bongkrekic acid D. oligomycin
D. oligomycin
Allows for uncoupling of ETC from ATP synthesis and dissipates the H+ gradient.
A. 2,4-DNP
B. Atractyloside
C. Bongkrekic acid
D. oligomycin
A. 2,4-DNP
Inhibits ATP-ADP translocase
A. 2,4-DNP
B. Atractyloside
C. oligomycin
B. Atracyloside
In addition to atractyloside, this also inhibits ATP-ADP translocase
bongkrekic acid
Add something about “Increased matrix ATP levels” and tie it back to the inhibition of ATP-ADP translocase
Okay
This is used to drive ATP Synthase
A. H+ gradient
B. ATP Synthase phosphorylase
C. UV radiation
D. ETC
A. H+ gradient
