Lecture 29 - Mycobacterial Infections II Flashcards
Names for ulcer caused by M ulcerans
Bairnsdale ulcer
Buruli ulcer
How was M ulcerans isolated from Bairnsdale ulcers?
Bacteriologists from the Alfred Hospital in Melbourne used a faulty incubator to incubate samples (couldn’t maintain 37C)
Optimal temperature at which M ulcerans grows
30C
Countries most affected by M ulcerans
Western and Central Aftrican nations
Mostly tropical regions
Number of M ulcerans cases in West Africa since 2000
Over 10,000
Demographic most affected by M ulcerans
Young children
M ulcerans treatment
Responds to combination therapy
Streptomycin, rifampicin for 8 weeks
No vaccine
M ulcernas mortality and morbidity
Doesn’t kill, but advanced cases can require surgery
M ulcerans epidemiology
1)
2)
3)
1) Very local epidemiology
2) Transmission appears to be from environment to humans (often aquatic environment)
3) Not human-human transmission
Point Lonsdale M ulcerans epidemic
1)
2)
3)
1) 3000 permanent residents
2) More than 90 cases since 2002
3) Nearby Queenscliff unaffected for a long time
M ulcerans pathology 1) 2) 3) 4) 5)
1) Prominent subcutaneous necrosis
2) Dermal layer of skin remains intact, tissue below dermis becomes encrotic
3) Ulcers of skin often painless
4) Granulomas often only form when ulcer begins to heal
5) An extracellular infection
Toxin produced by M ulcerans
Mycolactone
Mycolactone 1) 2) 3) 4)
1) A lipid toxin
2) Small, polyketide
3) Potent immunosuppressor at low concentrations
4) Cytotoxic at higher concentrations
Mycolactone- M ulcerans mutants
Avirulent
Pre-ulcerative lesion 1) 2) 3) 4) 5)
1) Small, movable nodule
2) Small bacterial load
3) Little tissue necrosis
4) Subcellular localisation
5) High local inflammatory response (IFNg)