Lecture 16 - Antimicrobial Mechanisms and Resistance II Flashcards
When are anti-membrane antimicrobials not used as a last resort?
In antifungal cases
Bonds linking rows of peptidoglycan
L-Lysine - Penta-glycine - D-Alanine
Polysaccharides making up peptidoglycan
N-acetylmuramic acid
N-acetylglucosamine
How are peptidoglycans cross-linked?
Penta-glycine displaces 2nd D-Ala on L/D polypeptide.
This is catalysed by transpeptidase
How is peptidoglycan assembled?
1)
2)
1) Building blocks are immobilised on the inside of the cell membrane
2) Assembled building blocks are flipped onto cell exterior
Other names for transpeptidases
Endopeptidases
Carboxypeptidases
When was vancomycin first used?
In the 1950s, when Staph aureus began to be resistant to beta-lactams.
What is MRSA?
Methicillin-resistant staph aureus
What does methicillin resistance signify?
Resistance to almost every other beta lactam
Which type of antimicrobial is vancomycin?
Glycopeptide
How does vancomycin work?
Binds directly to D-ala - D-ala on peptidoglycan precursor
Shape of vancomycin molecule
Very large, very charged
Why can’t vancomycin be used against G- bacteria?
Because it is very large and highly-charged
Can’t pass through thicker cell wall.
Which bacteria is vancomycin useful against?
Staph aureus
Bacteria innately resistant to vancomycin
Gram-, enterococci
Why are enterococci resistant to vancomycin?
D-ala - D-ala is replaced by D-ala - D-lac