Lecture 14 - More Membrane Secretion Flashcards

1
Q

What is GspE?

A

An ATPase in type II secretion system

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2
Q

Pilus secretion system similar to T2SS

A

Type 4 pilus

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3
Q

Type 4 pilus system
1)
2)
3)

A

Very similar to T2SS

1) PilA (pre-pilin) made with unusual signal peptide –> not recognised by Sec
2) Signal peptidase on CYTOPLASMIC side of membrane cleaves off signal sequence (PilD)
3) PilA secreted through complex very similar to T2SS

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4
Q

Prototypic T3SS

A

Yersinia YOP

pYV-encoded

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5
Q

Yop

A

In Yersinia
Contact-dependent T3SS
Normally switched off. Switched on when in contact with another cell.
Inserts proteins into cells that disrupt eukaryotic cell function

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6
Q

Unusual feature of type 4 pilus secretion

A

Pilus assembly occurs in periplasm

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7
Q

Bacterium that uses type 4 pili

A

Pseudomonas aeruginosa

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8
Q

Use of type 4 pili

A

Twitching motility

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9
Q

Type of bacteria that use T3SS

A

Pathogenic

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10
Q

Effects of Yop insertion on eukaryotic cells

A

Cytotoxicity
Inhibition of phagocytosis
Apoptosis
Inhibition of cytokine release

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11
Q

Yop pore that breaches host cell membrane

A

YopB/D pore

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12
Q

Yop B/D function

A

Membrane pore in host cell

Hydrophobic

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13
Q

Syc

A

Chaperone of proteins translocated in a T3SS

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14
Q

T3SS protein chaperone

A

Syc

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15
Q

Is Yop sec-dependent or independent

A

Independent

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16
Q

Is the N-terminal sequence of Yop proteins cleaved?

A

No

17
Q
Generic features of T3SS
1)
2)
3)
4)
5)
A

1) Needle structure that inserts proteins into target cell
2) Contact-dependent function
3) Sec-independent
4) No periplasmic stage
5) Only in pathogenic bacteria

18
Q

Which system has many homologous proteins to T3SS?

A

Flagellum secretion system

T3SS and flagellal basal body look very similar

19
Q

Type IV secretion system background

A

Very similar to bacterial conjugation systems

20
Q

1st protein secreted through a T3SS

A

Hydrophobic host cell membrane channel

21
Q

Which type of proteins can be translocated through T3SS needle?

A

Linear proteins

22
Q

Number of genes required for a T4SS

A

At least 10

23
Q

Best-studied T4SS

A

VirB11, VirB4 of Agrobacterium tumefaciens

24
Q

VirB4, VirB11 function

A

Part of Agrobacterium tumefaciens T4SS

Cytoplasmic ATPases which energise T4SS

25
Q

Cytoplasmic ATPases in Agrobacterium tumefaciens T4SS

A

VirB4, VirB11

26
Q

Uses of T4SS
1)
2)
3)

A

1) DNA transfer
2) DNA uptake
3) Virulence
4) Pilus formation (in A tumefaciens, for insertion of virulence proteins)

27
Q

Bacteria which use T4SS
1)
2)
3)

A

1) Legionella pneumophila
2) Bordatella pertussis
3) Helicobacter pylori

28
Q

What does pertussis use T4SS for?

A

Secretion of pertussis toxins

29
Q

2 different ways that proteins are trafficked through T4SS

A

1) With periplasmic stage. EG: pertussis toxin

2) Without periplasmic stage. EG: DNA, CagA

30
Q

How is pertussis toxin secreted
1)
2)
3)

A

1) Assembled as a preprotein with a typical signal peptide
2) Transferred through Sec
3) In periplasmic space enters T4SS, secreted

31
Q

T6SS

A

1) Not very well understood
2) Translocates proteins across bacterial envelope
3) Can insert proteins into target cell using system similar to bacteriophage cell-membrane puncturing protein

32
Q

Prototypical T6SS

A

Pseudomonas aeruginosa

33
Q

Which secretion system shares similarities with bacteriophage membrane-puncturing proteins?

A

T6SS

34
Q

T7SS

A

1) Newly discovered, not well understood
2) Transports proteins across mycobacterial cell wall
3) In G+ bacteria

35
Q

Name for mycobacterial T7SS

A

ESX-1 system

36
Q

In a TB infection, antibodies are produced against which part of T7SS?

A

CFP10, ESAT6