Lecture 14 - More Membrane Secretion Flashcards
What is GspE?
An ATPase in type II secretion system
Pilus secretion system similar to T2SS
Type 4 pilus
Type 4 pilus system
1)
2)
3)
Very similar to T2SS
1) PilA (pre-pilin) made with unusual signal peptide –> not recognised by Sec
2) Signal peptidase on CYTOPLASMIC side of membrane cleaves off signal sequence (PilD)
3) PilA secreted through complex very similar to T2SS
Prototypic T3SS
Yersinia YOP
pYV-encoded
Yop
In Yersinia
Contact-dependent T3SS
Normally switched off. Switched on when in contact with another cell.
Inserts proteins into cells that disrupt eukaryotic cell function
Unusual feature of type 4 pilus secretion
Pilus assembly occurs in periplasm
Bacterium that uses type 4 pili
Pseudomonas aeruginosa
Use of type 4 pili
Twitching motility
Type of bacteria that use T3SS
Pathogenic
Effects of Yop insertion on eukaryotic cells
Cytotoxicity
Inhibition of phagocytosis
Apoptosis
Inhibition of cytokine release
Yop pore that breaches host cell membrane
YopB/D pore
Yop B/D function
Membrane pore in host cell
Hydrophobic
Syc
Chaperone of proteins translocated in a T3SS
T3SS protein chaperone
Syc
Is Yop sec-dependent or independent
Independent