Lecture 2/Proteins

Question 1

general protein purification strategy by solubility

Answer 1

1. salting in

2. salting out

Question 2

General Protein Purification Strategy by ionic charge

Answer 2

1. ion exchange chromatography
2. electrophoresis
3. isoelectric focusing

Question 3

General Protein Purification Strategy by polarity

Answer 3

1. adsorption chromatography
2. paper chromatography
3. reverse-phase chromatography
4. hydrophobic interaction chromatography

Question 4

General Protein Purification Strategy by molecular size

Answer 4

1. dialysis and ultrafiltration
2. gel electrophoresis
3. gel filtration/size exclusion chromatography

Question 5

General Protein Purification Strategy by binding specificity

Answer 5

1. affinity chromatography

Question 6

to purify and concentrate biomolecules based on chemical or physical differences

Answer 6

chromatography