Furci

Question 1

nonpolar aliphatic R groups

Answer 1

glycine, alanine, valine, leucine, isoleucine

Question 2

aromatic R groups

Answer 2

phenylalanine, tyrosine, tryptophan

Question 3

sulfur containing R groups

Answer 3

cysteine and methionine

Question 4

aliphatic hydroxyl R groups

Answer 4

serine and threonine

Question 5

basic R groups

Answer 5

histidine, lysine, asparagine
polar and hydrophilic
positively charged

Question 6

acidic R groups

Answer 6

aspartic acid and glutamic acid
polar and hydrophilic
negatively charged

Question 7

amide derivatives

Answer 7

asparagine and glutamine

Question 8

proline shares properties with ____

Answer 8

nonpolar aliphatic R groups

Question 9

the process by which an atom or a molecule acquires a negative or positive charge by gaining or losing electrons to form ions, often in conjunction with other chemical changes.

Answer 9

ionization (ionization state)

Question 10

Describes the derivation of pH as a measure of acidity in biological and chemical systems
The equation is also useful for estimating the pH of a buffer solution
It is widely used to calculate the isoelectric point of proteins (point at which protein neither accept nor yield proton)

Answer 10

henderson hasselbach eqn

Question 11

the point at which the protein neither accepts or yields protons (molecule carries no net electric charge)

Answer 11

isoelectric point

Question 12

the linking together of ____ yields peptide chains

Answer 12

amino acids

Question 13

peptide bond is formed between the ____ of one amino acid and the ____ of the other; ____ is removed in the process

Answer 13

alpha-carboxyl; alpha-amino; water

Question 14

solubility

Answer 14

1. salting in

2. salting out

Question 15

ionic charge

Answer 15

1. ion exchange chromatography
2. electrophoresis
3. isoelectric focusing

Question 16

polarity

Answer 16

1. adsorption chromatography
2. paper chromatography
3. reverse-phase chr.
4. hydrophobic interaction chr.

Question 17

molecular size

Answer 17

1. dialysis and ultrafiltration
2. gel electrophoresis
3. gel filtration/size exclusion chr.

Question 18

binding specificity

Answer 18

1. affinity chr.

Question 19

to purify and concentrate biomolecules based on chemical or physical differences

Answer 19

chromatography

Question 20

chroma. based on size

Answer 20

size exclusion chroma. (SEC) AKA gel filtration chroma

Question 21

chroma. by surface charge

Answer 21

ion exchange chroma

Question 22

chroma by biorecognition (ligand specificity)

Answer 22

affinity chroma

Question 23

types of SEC

Answer 23

dialysis and ultrafiltration; also gel filtration

Question 24

in gel filtration, ____ molecules pass through quickly and ____ molecules pass through slowly

Answer 24

large; small

Question 25

anion exchanger is ____ charged

Answer 25

positively; attracts negative things

Question 26

cation exchanger is ___ charged

Answer 26

negatively; attracts positive things

Question 27

some ways to characterize proteins by separation methods

Answer 27

1. buffer exchange and concentration
2. electrophoresis and discontinued gel electrophoresis
3. SDS-PAGE
4. isoelectric focusing: based on pI
5. characterization assays

Question 28

Electron transport system accepts energy from carriers in the ____ and stores it to a form that can be used to phosphorylate ____

Answer 28

matrix; ADP

Question 29

two energy carriers of ETS

Answer 29

nicotine adenine dinucleotide (NAD)

flavin adenine dinucleotide (FAD)

Question 30

complex I

Answer 30

NADH-Q reductase complex

Question 31

complex II

Answer 31

succinate complex ; is a separate starting point and is not part of the NADH pathway

Question 32

complex III

Answer 32

cytochrome c reductase

Question 33

complex IV

Answer 33

cytochrome c oxidase

Question 34

complex V

Answer 34

ATP synthase complex