Lecture 18-19 (Genetic Tools) Flashcards
Trying to determine the unknown genotype (gene responsible) of an known phenotype (physical characteristic) of a bacterium would be Forward genetics.
T/F
True
If you were checking bacterial isolates for acquired antibiotic resistance by plated them on growth media with antibiotics (i.e. what you want will survive), this would be a type of what?
A. Negative selection
B. Positive selection
C. None of these
D. Differential screen
B.
If you were checking bacterial isolates for lost antibiotic resistance by plating them on growth media with antibiotics (i.e. what you want would die), this would be a type of what?
A. Negative selection
B. Differential selection
C. Positive selection
D. None of these
A.
LacZ and GFP are examples of reported proteins, these is typically used for what?
A. Negative selection
B. Positive selection
C. None of these
D. Differential screen
D.
A recombinant plasmid is a bacterial plasmid that has DNA from another species inserted into it.
T/F
True
Heat shock and electroporation are variants of what bacterial process?
A. Conjugation
B. Transduction
C. Transformation
D. Recombination
C.
Using homologous recombination for genomic engineering/mutagenesis can only be performed using plasmids.
T/F
False
What bacterial protein is essential for bacterial recombination?
A. RNA polymerase
B. RecA
C. DNA polymerase IV
D. Noc
B.
Which of these proteins are not part of the CRISPR-Cas9 system?
A. RecA
B. tracrRNA
C. Protospacers
D. Cas Proteins
A.
What natural repair mechanism in eukaryotes is utilized with CRISPR-Cas to allow for targeted mutation?
A. recombination
B. Transformation
C. Non-homologous end joining
D. Sexual Reproduction
C.
The difference between Transcriptional Fusions and Translational Fusions, in reference to protein synthesis, is?
Transcriptional Fusions has 2 separate proteins with one have the desired gene phenotype, while Translational Fusions have fused proteins where you can see where the desired gene expression begins.
